Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz

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Gewählte Publikation:

Quan, P.
In vivo effects of norepinephrine retard tablets on biological properties of haematopoietic stem cells (HSCs) in rats
[ Dissertation ] Medical University of Graz; 2007. pp.

 

Autor/innen der Med Uni Graz:
BetreuerInnen:
Dohr Gottfried
Preisegger Karl-Heinz
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Abstract:
In the present study the in vivo influence of enhanced peripheral norepinephrine (NE) on immune cells and haematopoiectic stem cells (HSCs) and/or haematopoietic progenitor cells (HPCs) derived from the peripheral blood (PBL), spleen and bone marrow (BM) was investigated with 12h or 24h subcutaneous implantation of NE retard tablets in rats. The main conclusions can be summarized as following: The sustained enhanced plasma NE level induced significantly peripheral immune responses, including profound reductions in splenic cellularity, leukocytosis whit a shift in the differential circulation leukocyte counts, i.e., an increase in neutrophils and a drop in total lymphocytes, and a marked suppression of the in vitro responsiveness of PBL lymphocytes. Besides the noticeable alterations in the peripheral immune system, the long-term NE treatment has great impacts on HSCs/HPCs derived from the PBL, spleen and BM. The 12h and 24h NE treatment promoted the mobilization of HSCs/HPCs from the BM into circulation, accompanied by an inhibited in vitro growth of HSCs, HPCs in the 24h NE treated rats. The 24h NE treatment resulted in a significant decrease in the number and the in vitro growth of splenic HSCs/HPCs. Meanwhile, the 24h NE treatment led to an accumulation of splenic MNCs in G0/G1-phase. The NE treatment has no marked effect on the frequency and the in vitro growth of HSCs/HPCs derived from the BM. However, the 24h NE treatment caused a decline in the self-renewal capacity of BM HSCs/HPCs. The functions of NE are mainly mediated with increasing oxide stress levels.

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