Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Blanz, P; Buzina, W; Ginter, G; Gräser, Y.
Molecular biological methods and their consequences in taxonomy and diagnosis of dermatophytes
Mycoses. 2000; 43 Suppl 1:11-16
Web of Science
PubMed
FullText
- Co-Autor*innen der Med Uni Graz
- Buzina Walter
- Ginter-Hanselmayer Gabriele
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Abstract:
- Trichophyton rubrum, T. mentagrophytes, T. verrucosum, Microsporum canis, M. gypseum and Epidermophyton flocoosum represent the cause of human dermatomycoses isolated most often at the University Hospital of Dermatology in Graz, Austria, between 1991 and 1998. So far, identification was mainly based on the cultivation of fungal isolates on special media as well as on the analysis of their microscopic characters. Restriction enzyme length polymorphisms (RFLPs) were now used to identify these human fungal parasites. For this purpose, internal transcribed spacers (ITS) which are located between ribosomal RNA genes have been amplified by using PCR and have afterwards been used to generate species specific RFLP patterns. By this method, a fast and reliable identification of these species was made possible. Nucleotide sequence data of this region not needed for identification have been worked out to show RFLPs in more detail.
- Find related publications in this database (using NLM MeSH Indexing)
- Arthrodermataceae - classification
- Austria - classification
- DNA, Ribosomal - genetics
- Dermatomycoses - diagnosis
- Genes, Fungal - diagnosis
- Humans - diagnosis
- Polymorphism, Restriction Fragment Length - diagnosis
- Restriction Mapping - diagnosis
- Find related publications in this database (Keywords)
- Trichophyton
- Microsporum
- Epidermiphyton
- Arthroderma
- polymerase chain reaction
- PCR restriction enzyme length polymorphism
- RFLP
- identification