Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Frohn, C; Höppner, M; Schlenke, P; Kirchner, H; Koritke, P; Luhm, J.
Anti-myeloma activity of natural killer lymphocytes.
Br J Haematol. 2002; 119(3):660-664
Doi: 10.1046/j.1365-2141.2002.03879.x
Web of Science
PubMed
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- Co-Autor*innen der Med Uni Graz
- Schlenke Peter
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- Abstract:
- Natural killer (NK) cells are assumed to contribute to a graft-versus-leukaemia effect. In vitro experiments have shown that many leukaemic cells are NK-cell sensitive. Nevertheless, no data concerning the influence of purified NK cells on malignant myeloma (MM) cells exist. We co-incubated NK cells with three different MM cell lines and fresh bone marrow samples of nine MM patients. The proportion of vital MM cells was determined before and after co-cultivation by a flow-cytometry-based assay. All MM cells tested, with the exception of one cell line (NCI H929), were susceptible to a NK-cell attack even without exogenous interleukin 2 (IL-2). The mean killing of the native MM samples was 23.1 +/- 5.4% and 34.5 +/- 6.5% at 10:1 and 20:1 effector:target ratio respectively, This corresponded to about 2/3 of those values obtained with the highly sensitive line K562. In contrast, CD34-positive haematopoietic stem cells as well as peripheral mononuclear cells were completely resistant under similar experimental conditions (1.3% killing). To elucidate the underlying triggering mechanisms, we measured human leucocyte antigen (HLA)-class I expression of the MM cells. No evidence for HLA loss, which could have explained the NK-cell recognition if it occurred, was demonstrated. These findings may contribute to the understanding of in vivo NK-cell activation and encourage clinical applications of NK cells for MM patients.
- Find related publications in this database (using NLM MeSH Indexing)
- Cytotoxicity, Immunologic - immunology
- Flow Cytometry -
- Genes, MHC Class I - immunology
- Graft vs Leukemia Effect - immunology
- Humans -
- Killer Cells, Natural - immunology
- Multiple Myeloma - immunology
- Tumor Cells, Cultured -
- Find related publications in this database (Keywords)
- natural killer cells
- multiple myeloma
- cellular cytotoxicity
- HLA expression
- GVL effect