Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Michenthaler, H.
Multi-omics approaches to characterize acute and cyclic fasting responses
Doktoratsstudium der Medizinischen Wissenschaft; Humanmedizin; [ Dissertation ] Medizinische Universität Graz; 2025. pp.
- Autor*innen der Med Uni Graz:
- Betreuer*innen:
- Krstic Jelena
- Prokesch Andreas
- Sedej Simon
- Altmetrics:
- Abstract:
- Fasting activates a coordinated systemic response involving key metabolic tissues, like adipose tissue (AT) and liver. At the onset of fasting, AT rapidly mobilizes stored lipids, while the liver initiates glycogenolysis followed by gluconeogenesis and ketogenesis to maintain long-term energy homeostasis. Key players in these adaptations are transcription factors (TFs) that bind to promoter and enhancer regions to coordinate cellular transcriptional programs. Although individual fasting-responsive regulators have been described, their time-resolved coordinated activity remains not fully understood. Transcriptionally active TF-bound enhancers generate short-lived enhancer RNAs (eRNAs). Using quantitative precision run-on sequencing (qPRO-seq) we generated the first nascent transcription-based atlas of fasting-regulated genes and enhancers in mouse liver and AT.