Medizinische Universität Graz - Research portal
Selected Publication:
Fruhwirt, L.
Investigating the metabolic function of Nr4a1 in aggressive lymphomas.
[ Diplomarbeit/Master Thesis (UNI) ] Universität Graz; 2025.
- Authors Med Uni Graz:
- Advisor:
- Deutsch Alexander
- Altmetrics:
- Abstract:
- Lymphomas are a heterogeneous group of malignant diseases whose cellular metabolism is significantly reprogrammed to maintain uncontrolled proliferation. These metabolic alterations are characterized by altered expression of glycolysis-associated enzymes, increased glucose uptake, increased glutamine consumption, the ability to utilize both glycolytic and oxidative metabolism and enhanced fatty acid synthesis. These metabolic adaptations contribute significantly to tumorigenesis, disease progression, and chemotherapy resistance. Metabolic remodelling encompasses processes of glucose, nucleic acid, amino acid, and lipid metabolism and is influenced not only by genetic and epigenetic alterations but also by microecological factors such as viral infections. The aim of this study was to investigate the metabolic differences of mouse lymphoma cells with different genotypes. Four different cell lines were used for this study: EµMyc Nr4a1 +/+ and EµMyc Nr4a1 -/- cells, each in the IgM⁺ and IgM⁻ lymphoma cell variants. The results of the analysis show that Nr4a1 knockout leads to increased activation of the purine salvage pathway, increased de novo synthesis of pyrimidines, and has effects on the TCA cycle, the urea cycle, and one-carbon metabolism. Furthermore, more pronounced metabolic changes were observed after Nr4a1 knockout, particularly in undifferentiated B cells.