Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Payr, S.
Regulation of lipolysis in the human term placenta by maternal obesity
Humanmedizin; [ Diplomarbeit ] Graz Medical University; 2016. pp.
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- Autor*innen der Med Uni Graz:
- Betreuer*innen:
- Wadsack Christian
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- Abstract:
- Background: Knowledge of consequences of maternal obesity in human placental fatty acid (FA) transport is limited. Animal studies suggest that placental FA-uptake is altered by maternal over-nutrition. Catabolism of FA storage depots and mobilization of free fatty acids (FFA) in the placenta depends on lipases. Placental FA metabolism is tightly regulated by maternal lipolytic activities. Hypothesis: Maternal pre-pregnancy body mass index (BMI) affects human placental FA transport by modifying expression of key genes. The impact of maternal over-nutrition on functionality of the feto-placental unit was investigated. Methods: Term placentas from normal (BMI 18.5–24.9 kg/m2) and obese (BMI > 30 kg/m2) women were analysed. Based on NanoString® generated results from placental tissues, adipose triglyceride lipase (ATGL) and its co-factors comparative gene identification 58 (CGI-58) and G0/G1-switch gene 2 (G0S2) were identified as key genes in lipolysis together with perilipin 3 (PLIN3), angiopoetin-like protein 4 (ANGPTL4), sterol O-acetyltransferase 1 and 2 (SOAT1/SOAT2). Correlations of obesity dependent placental lipid associated genes with maternal and neonatal available clinical parameters were calculated. Additionally, using placental tissue, qRT-PCR and Western blot were employed, to confirm global genomic data on RNA and protein levels in placentas from highly obese mothers compared to controls. Results: Degree of maternal obesity was not related to ATGL mRNA levels in the studied placentas. The ATGL co-activator and lipid droplet binding protein CGI-58 however, correlated positively with maternal BMI. No association could be detected between CGI-58- and ATGL-levels. Influence of CGI-58 on ATGL enzyme activity was not tested in this study. Conclusions: Placental lipolysis appears to be altered by alternated CGI-58 levels in maternal obesity.