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Selected Publication:
Biebl, E.
Chronische Wunden - Enzymbasierte Diagnosemöglichkeit infizierter Wundflüssigkeiten
[ Diplomarbeit ] Graz Medical University; 2012. pp. 80
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- Authors Med Uni Graz:
- Advisor:
- Schintler Michael
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- Abstract:
- Purpose of the study Diagnosis of wound infection is based on the well known ¿traditional signs of inflammation¿ like rubor (redness), calor (heat), tumor (swelling) and dolor (pain) by experienced doctors and/or resource consuming and expensive procedures. At present there is no rapid diagnostic device on the market for the detection of wound infection which would especially be helpful in home care of chronic ulcer patients [1] . Material and methods In this study, a new concept for a fast diagnostic tool for wound infection based on lysozyme and elastase triggered release of dye from a peptidoglycane matrix was investigated. The matrix consisted of alginate/agarose and peptidoglycane covalently labelled with Remazol Brilliant Blue (RBB). Lysozyme activity in post operative wounds and decubitus wound fluids was significantly elevated upon infection (4,830 + 1,848 U mL-1) compared to non infected wounds (376 + 240 U mL-1) [1]. Results : Consequently, incubation of 8% (w/v) labelled agarose/peptidoglycane blend layers with infected wound fluid samples for 2 hours at 37°C resulted in a 4 fold higher amount of dye released than measured for non-infected wounds. For alginate/peptidoglycane beads a 7 fold higher amount of dye was released in case of infected wound fluid samples compared to not-infected ones. Apart from lysozyme, proteases (i.e. gelatinase MMP-2 and MMP-9 and elastase) were detected in wound fluids (e.g. using Western plotting). When dosed in ratios typical for wounds, a slight synergistic effect was measured for peptidoglycane hydrolysis (i.e. dye release) between lysozyme and these proteases [1]. Conclusion: Incubation of a double layer system consisting of stained and non-stained peptidoglycane with infected wound fluids resulted in a colour change from yellow to blue thus allowing simple visual detection of wound infection