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Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Khoschsorur, G; Erwa, W; Fruehwirth, F; Stettin, M; Meinitzer, A; Hoebarth, G; Zelzer, S; Halwachs-Baumann, G.
High-performance liquid chromatographic method for the simultaneous determination of cyclosporine A and its four major metabolites in whole blood.
Talanta. 2005; 65(3):638-643
Doi: 10.1016/j.talanta.2004.07.048
Web of Science
PubMed
FullText
FullText_MUG
- Leading authors Med Uni Graz
- Khoschsorur Gholamali
- Co-authors Med Uni Graz
- Baumann Gabriele
- Erwa Wolfgang
- Fruehwirth Franz
- Hoebarth Gerhard
- Meinitzer Andreas
- Stettin Mariana
- Zelzer Sieglinde
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- Abstract:
- This study aimed to develop a simple and efficient optimized high-performance liquid chromatograph (HPLC) method for simultaneous determination of cyclosporine A (CyA) and its major, partly active metabolites AM1, AM9, AM4N, and AM19 in whole blood from transplant patients using cyclosporine D (CyD) as internal standard. The method used a CN analytical column maintained at 60 degrees C with hexan-isopropanol (93:7, v/v) as mobile phase; detection was at 212nm. Linearity for all five compounds was tested in the range of 31-1500ngml(-1) for CyA and of 31-1000ngml(-1) for metabolites. The limit of detection was found to be 15ngml(-1) for all compounds. This modified, inexpensive method is also suitable for measuring cyclosporine A and metabolite concentrations in routine monitoring of patients undergoing treatment with CyA.
- Find related publications in this database (Keywords)
- cyclosporine A
- metabolites (AMI
- AM9
- AM4N
- AM19)
- HPLC