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Selected Publication:
GRAIER, WF; SCHMIDT, K; KUKOVETZ, WR.
ACTIVATION OF G-PROTEIN EVOKES CA2+ INFLUX IN ENDOTHELIAL-CELLS WITHOUT CORRELATION TO INOSITOL PHOSPHATES
J CARDIOVASC PHARMACOL. 1991; 17: S71-S78.
Doi: 10.1097/00005344-199117003-00013
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- Graier Wolfgang
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- Abstract:
- The production of big endothelin-1 (big ET-1) and its processing to endothelin (ET-1) were examined in cocultures of bovine pulmonary artery endothelial cells (BPECs) and human bronchiolar smooth muscle cells (HBSMCs). The BPECs produced immunologically reactive ET-1 (ir-ET-1) and big ET-1 (ir-big ET-1) that increased linearly in the cell culture media over a 72 h time course when confluent monolayers were incubated at 37 degrees C in serum-free media. HBSMCs maintained in serum-free media over a 72-h period did not produce any detectable ir-big ET-1 or ir-ET-1. After coculture of these two cell types for 24 h (the monolayers were physically separated), the culture medium contained no detectable ir-big ET-1 or ir-ET-1. When medium conditioned for 24 h with the HBSMCs was transferred to the BPEC monolayers, the accumulation rates of ET-1 and big ET-1 were attenuated 70 and 10%, respectively. When medium conditioned for 24 h with the BPECs was transferred to the HBSM cell monolayers, the amount of ir-ET-1 and ir-big ET-1 in the medium decreased greater than 90% over the next 24 h. Cellular binding and uptake could not account for this decrease as ascertained by quantitation of cellular levels of ET-1 and big ET-1. When conditioned medium from the HBSMCs was added to conditioned medium from the BPECs in a 1:1, 2:1, 3:1, 1:2, or 1:3 ratio (v/v) in the absence of cells, the amount of ET-1 or big ET-1 present in the EC medium did not decrease significantly over the following 24 h. These data imply that HBSMCs contain a factor that is responsible for decreasing the accumulation of ir-big ET-1 and ir-ET-1 in the cell culture media of BPECs. The data also suggest that the most likely mechanism for this effect is a factor that degrades BET-1 and ET-1 at different rates.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Bronchi - metabolism
- Cattle -
- Cells, Cultured -
- Culture Media -
- Endothelin-1 -
- Endothelins - metabolism
- Endothelium, Vascular - metabolism
- Humans -
- Muscle, Smooth - metabolism
- Protein Precursors - metabolism
- Pulmonary Artery - metabolism
- Radioimmunoassay -
- Find related publications in this database (Keywords)
- ENDOTHELIAL CELL
- G-PROTEIN
- ENDOTHELIUM-DERIVED RELAXING FACTOR (EDRF)
- CA2+ INFLUX
- SODIUM FLUORIDE
- IP3
- IP4