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Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Majer, S; Bauer, M; Magnet, E; Strele, A; Giegerl, E; Eder, M; Lang, U; Pertl, B.
Maternal urine for prenatal diagnosis--an analysis of cell-free fetal DNA in maternal urine and plasma in the third trimester.
Prenat Diagn. 2007; 27(13):1219-1223
Doi: 10.1002/pd.1875
Web of Science
PubMed
FullText
FullText_MUG
- Leading authors Med Uni Graz
- Pertl Barbara
- Reisinger Sandra
- Co-authors Med Uni Graz
- Eder Martina
- Giegerl Elisabeth
- Groselj-Strele Andrea
- Holzapfel-Bauer Margit
- Lang Uwe
- Magnet Eva Maria Sieglinde
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- Abstract:
- The aim of the study was the detection, quantification and correlation of cell-free fetal (cff) DNA in maternal urine and plasma in normal and complicated pregnancies during the third trimester. One hundred and fifty-one urine and plasma samples obtained from 96 women carrying male fetuses, and 55 carrying female fetuses were collected and analyzed for cff-DNA using fluorescent PCR and quantitative real-time PCR. DNA was extracted from 1 mL maternal urine and analyzed with two different primer sets (SRY and DYS-14). The concentrations of cff and total DNA in maternal plasma were correlated with maternal and obstetric parameters using appropriate correlation analyses. Y-chromosome-specific sequences were detected in 31 of 96 (32.3%) urine samples collected from women pregnant with male fetuses using DYS-14 and in 6 of 96 (6.3%) urine samples using SRY as primers using real-time PCR. All 96 plasma samples obtained from women carrying male fetuses were positive for cff-DNA using real-time PCR. Cff-DNA exhibited a correlation with gestational age (R = 0.244; P = 0.018) and an inverse correlation with the latency between blood collection and birth (R = - 0.218; P = 0.036). Total DNA showed a correlation with placental weight (R = 0.182; P = 0.034) and pregnancy-associated complications (R = 0.280; P < 0.001). Our data confirm that cff-DNA is cleared by the kidneys in detectable amounts, but due to its low concentration or problematic detection in maternal urine this source seems inappropriate for noninvasive prenatal diagnosis. Copyright (c) 2007 John Wiley & Sons, Ltd.
- Find related publications in this database (using NLM MeSH Indexing)
- Aneuploidy -
- Biomarkers - blood
- Chromosomes, Human, Y - genetics
- DNA - urine
- Female -
- Fetus -
- Humans -
- Male -
- Maternal-Fetal Exchange - genetics
- Polymerase Chain Reaction - methods
- Pregnancy -
- Pregnancy Trimester, Third -
- Prenatal Diagnosis - methods
- Tandem Repeat Sequences -
- Urinalysis - methods
- Find related publications in this database (Keywords)
- cff-DNA
- maternal urine and plasma
- third trimester
- fluorescent PCR
- real-time PCR