Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Saria, A; Mayer, N; Lembeck, F; Pabst, M.
Regional distribution and biochemical properties of 125I-Tyr8-substance P binding sites in synaptic vesicles.
NAUNYN-SCHMIED ARCH PHARMACOL. 1980; 311(2): 151-157.
Doi: 10.1007/BF00510254
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- Co-Autor*innen der Med Uni Graz
- Lembeck Fred
- Pabst Maria-Anna
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- Abstract:
- 1. Binding of 125I-Tyr8-substance P (SP) to synaptic vesicles shows an uneven distribution within the brain and the spinal cord. The regional distribution has a positive correlation with the SP-content, except in the hypothalamus. 2. Ca2+ and MG2+-ions (1 and 10 mM) decrease the number of binding sites without alteration of affinity. EDTA and EGTA enhance SP-binding which is interpreted as being due to removal of the inhibitory influence of endogenous Ca2+ and Mg2+ through chelation with these agents. No significant inhibition of SP binding was observed by Na+ or K+ in concentrations below 100 mM. 3. Pretreatment of synaptic vesicles with trypsin or with phospholipase A2, C and D leads to a total loss of SP binding showing a proteolipid or a joint protein-phospholipid nature of these binding sites. SH groups do not contribute to SP binding since no effect of N-ethylmaleimide and monoidoacetic acid on SP binding was found.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
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- Membranes - metabolism
- Phosphorus - metabolism
- Proteins - metabolism
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- Substance P - immunology
- Sulfhydryl Compounds - pharmacology
- Synaptic Vesicles - metabolism