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Lind, P; Lechner, P; Hausmann, B; Smola, MG; Koeltringer, P; Steindorfer, P; Cesnik, H; Passl, R; Eber, O.
Development of Human Antimouse Antibodies (Hama) After Single and Repeated Diagnostic Application of Intact Murine Monoclonal-Antibodies
ANTIBODY IMMUNOCONJ RADIOPHAR. 1991; 4: 811-818.
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Co-authors Med Uni Graz: Smola Michael
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Abstract:: The HAMA response after diagnostic application of murine monoclonal antibodies was examined in 67 patients. In 23 cases HAMA's were determined before and three months after administration of 1 mg intact monoclonal anti-CEA antibody (MAb BW 431/26), in 29 patients before and three months after application of 0.5 mg intact monoclonal antigranulocyte antibody (MAb BW 250/183). In another group of 15 patients, HAMA's were determined at three-week intervals before and after repeated application of 0.3 mg MAb BW 250/183. In all patients total HAMA response (IgM and IgG) as well as the proportion of antiidiotypic response (IgM and IgG) was determined by an enzyme immuno assay (Enzygnost HAMA micro, Behring, Marburg FRG). After single application of 1 mg MAb BW 431/26, 7 out of 23 patients developed HAMA's (6 patients with predominantly antiisotypic, 1 patient with predominantly antiidiotypic response). After administration of 0.5 mg MAb BW 250/183, 4 out of 29 patients developed HAMA's (3 patients with predominantly antiisotypic, 1 patient with predominantly antiidiotypic response). At repeated application of 0.3 mg MAb BW 250/183 none of the 15 patients developed human antimouse antibodies after the first application; after the second and third application one patient showed a clear increasing antiidiotypic response one patient a marginal antiidiotypic response, two patients an antiisotypic response and 4 patients a transient predominantly antiisotypic IgM response. Deterioration of image quality in repeated immunoscintigraphic investigations was observed in cases of high HAMA titers and/or predominantly antiidiotypic HAMA response.