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Selected Publication:
Brunner, F; Stessel, H; Simecek, S; Graier, W; Kukovetz, WR.
Effect of intracellular Ca2+ concentration on endothelin-1 secretion.
FEBS Lett. 1994; 350(1):33-36
Doi: 10.1016/0014-5793(94)00727-6
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- Co-authors Med Uni Graz
- Graier Wolfgang
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- Abstract:
- The role of intracellular free Ca2+ concentration ([Ca2+]i) in cellular regulation of endothelin-1 (ET-1) secretion was investigated in cultured porcine aortic endothelial cells of first passage. Intracellular Ca2+ concentrations were adjusted between 50 nM and 1 microM using EGTA and thapsigargin, respectively. ET-1 secretion was maximal at [Ca2+]i of 190-470 nM, and reduced at low (50 and 110 nM) and high (> 470 nM) [Ca2+]i. The Ca2+ ionophores A23187 and ionomycin (each 1 microM), both of which raise [Ca2+]i above 1 microM, also potently inhibited ET-1 secretion under basal and stimulated conditions. The A23187-induced reduction in ET-1 secretion was not affected by NG-nitro-L-arginine (0.1 mM). Our results provide evidence that basal ET-1 secretion is regulated by Ca2+ and that Ca2+ ionophores reduce ET-1 secretion due to the inhibitory effect of high [Ca2+]i.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Calcimycin - pharmacology
- Calcium - metabolism
- Cells, Cultured - metabolism
- Endothelins - secretion
- Endothelium, Vascular - drug effects
- Ionomycin - pharmacology
- L-Lactate Dehydrogenase - metabolism
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- Find related publications in this database (Keywords)
- ENDOTHELIN-1 SECRETION
- CYTOSOLIC FREE CA2+
- CULTURED ENDOTHELIAL CELL
- CA2+ IONOPHORE
- NITRIC OXIDE SYNTHASE INHIBITOR
- PORCINE AORTA