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März, W; Baumstark, MW; Scharnagl, H; Ruzicka, V; Buxbaum, S; Herwig, J; Pohl, T; Russ, A; Schaaf, L; Berg, A.
Accumulation of "small dense" low density lipoproteins (LDL) in a homozygous patients with familial defective apolipoprotein B-100 results from heterogenous interaction of LDL subfractions with the LDL receptor.
J Clin Invest. 1993; 92(6):2922-2933 Doi: 10.1172/JCI116915 (- Case Report) [OPEN ACCESS]
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Leading authors Med Uni Graz: März Winfried
Co-authors Med Uni Graz: Scharnagl Hubert
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Abstract:: The interaction of LDL and LDL subfractions from a patient homozygous for familial defective apoB-100 (FDB) has been studied. His LDL cholesterol ranged from 2.65 to 3.34 g/liter. In cultured fibroblasts, binding, internalization, and degradation of the patient's LDL was diminished, but not completely abolished. The patient's apolipoprotein E concentration was low, and the amount of apolipoprotein E associated with LDL was not elevated over normal. LDL were separated into six subfractions: LDL-1 (1.019-1.031 kg/liter), LDL-2 (1.031-1.034 kg/liter), LDL-3 (1.034-1.037 kg/liter), LDL-4 (1.037-1.040 kg/liter), LDL-5 (1.040-1.044 kg/liter), and LDL-6 (> 1.044 kg/liter). LDL-5 and LDL-6 selectively accumulated in the patient's plasma. Concentrations of LDL-1 to 3 were normal. The LDL receptor-mediated uptake of LDL-1 and LDL-2 could not be distinguished from normal LDL. LDL-3 and LDL-4 displayed reduced uptake; LDL-5 and LDL-6 were completely defective in binding. When apolipoprotein E-containing particles were removed by immunoabsorption before preparing subfractions, LDL-3 and LDL-4, but not LDL-1 and LDL-2, retained some receptor binding activity. We conclude that in FDB, LDL-1 and LDL-2 contain sufficient apolipoprotein E to warrant normal cellular uptake. In LDL-3 and LDL-4, the defective apoB-100 itself displays some receptor binding; LDL-5 and LDL-6 are inable to interact with LDL receptors and accumulate in plasma.
Find related publications in this database (using NLM MeSH Indexing): Adolescent -; Adult -; Apolipoprotein B-100 -; Apolipoproteins B - genetics; Base Sequence -; Child -; Cholesterol - blood; DNA Primers -; Female -; Fibroblasts - metabolism; Heterozygote Detection -; Homozygote -; Humans -; Hyperlipoproteinemia Type II - drug therapy; Lipoproteins - blood; Lipoproteins, LDL - genetics; Male -; Middle Aged -; Molecular Sequence Data -; Pedigree -; Polymerase Chain Reaction -; Pravastatin - therapeutic use; Receptors, LDL - metabolism; Skin - metabolism; Triglycerides - blood