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Selected Publication:
März, W; Siekmeier, R; Scharnagl, H; Seiffert, UB; Gross, W.
Fast lipoprotein chromatography: new method of analysis for plasma lipoproteins.
Clin Chem. 1993; 39(11 Pt 1):2276-2281
Doi: 10.1093/clinchem/39.11.2276
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- Leading authors Med Uni Graz
- März Winfried
- Co-authors Med Uni Graz
- Scharnagl Hubert
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- Abstract:
- Fast lipoprotein chromatography (FLPC) is a novel method for quantifying lipoproteins. Plasma proteins are separated by fast-flow gel filtration. Lipoproteins are detected by post-column derivatization with an enzymatic cholesterol reagent. FLPC resolves very-low-, low-, and high-density lipoproteins (VLDL, LDL, and HDL, respectively) and completely separates apolipoprotein Al- and apolipoprotein B-containing lipoproteins. CVs for VLDL-cholesterol, LDL-cholesterol, and HDL-cholesterol are 5.8%, 2.0%, and 1.9%, respectively. We compared FLPC with a combined ultracentrifugation and precipitation method and obtained correlation of r = 0.979, 0.978, and 0.933 for VLDL-cholesterol, LDL-cholesterol, and HDL-cholesterol, respectively. Triglyceride concentrations up to 9.00 g/L did not interfere with the quantification of lipoproteins by FLPC. We conclude that FLPC is a precise and reliable method for the analysis of plasma lipoproteins that complements conventional techniques.
- Find related publications in this database (using NLM MeSH Indexing)
- Apolipoprotein A-I - isolation and purification
- Apolipoproteins B - isolation and purification
- Chromatography, High Pressure Liquid - methods
- Comparative Study - methods
- Humans - methods
- Hyperlipoproteinemia - blood
- Lipoproteins - blood
- Lipoproteins, HDL - blood
- Lipoproteins, HDL Cholesterol - blood
- Lipoproteins, LDL - blood
- Lipoproteins, LDL Cholesterol - blood
- Lipoproteins, VLDL - blood
- Lipoproteins, VLDL Cholesterol - blood
- Research Support, Non-U.S. Gov't - blood
- Find related publications in this database (Keywords)
- CHOLESTEROL
- HYPERLIPOPROTEINEMIA
- APOLIPOPROTEINS
- GEL PERMEATION CHROMATOGRAPHY
- ULTRACENTRIFUGATION
- INTERMETHOD COMPARISON