Medizinische Universität Graz - Research portal
Selected Publication:
Schauentstein, E; Nöhammer, G; Bajardi, F; Urdl, W.
Possibilities and limits of the quantitative demonstration of protein thiols with the DDD-reagent in cells of cervical epithelium (author's transl)
J Cancer Res Clin Oncol. 1981; 100(2): 225-228.
Doi: 10.1007/BF00403369
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- Co-authors Med Uni Graz
- Urdl Wolfgang
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- Abstract:
- From six patients, all with invasive carcinoma of the cervix uteri, two groups fo smear preparations, each consisting of eight samples, were taken: The first, group A, before and the second, group B, after mechanical removal of the discharge which was present in abundant quantities. All samples were treated for 24 h with DDD to reach the fast-reaching SH-groups, coupled with Fast Blue B and measured cytophotometrically at 560 nm. Without any exception, the neoplastic cells of group A showed significantly lower extinction values both of the nucleus and of the total cell, if compared to group B. The mean extinction difference amounts to 55% and is highly significant and some evidence is given that the viability of the cells plays an important role. For this reason, the quantitative evaluation of the DDD Fast Blue B does not seem to be useful for cytologic routine investigations.
- Find related publications in this database (using NLM MeSH Indexing)
- Cervix Uteri - analysis
- Disulfides - diagnostic use
- English Abstract - diagnostic use
- Epithelium - analysis
- Female - analysis
- Humans - analysis
- Naphthols - diagnostic use
- Neoplasm Proteins - analysis
- Sulfhydryl Compounds - analysis
- Sulfhydryl Reagents - diagnostic use
- Uterine Cervical Neoplasms - analysis