Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Dohr, G; Blaschitz, A; Hirsch, T; Kohlstädt, S; Uchanska-Ziegler, B; Ziegler, A.
Immunoelectron microscopy of human spermatozoa.
ANDROLOGIA . 1990; 22 Suppl 1(5): 92-100.
Doi: 10.1111/j.1439-0272.1990.tb02075.x
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PubMed
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- Führende Autor*innen der Med Uni Graz
- Dohr Gottfried
- Co-Autor*innen der Med Uni Graz
- Blaschitz Astrid
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- Abstract:
- A number of immunocytochemical methods using various markers for electron microscopy have been developed in recent years. The immunogold technique has been especially effective in histotopochemical studies. The value of this technique for demonstrating sperm antigens results from the high electron density of gold, which makes it easily detectable under the electron microscope. The high resolution of the electron microscope permits precise localization of immunologic reactions in the sperm cell. Light microscopy findings can thus be elucidated. We tested a number of monoclonal antibodies that react with sperm antigens. Of three techniques for preparing the spermatozoa, the pre-embedding method and marking of cryoultra-microtome sections proved best.
- Find related publications in this database (using NLM MeSH Indexing)
- Fluorescent Antibody Technique -
- Humans -
- Immunohistochemistry -
- Male -
- Microscopy, Immunoelectron -
- Spermatozoa - ultrastructure
- Tissue Embedding - methods
- Find related publications in this database (Keywords)
- Human Sperm Cells
- Immunoelectron Microscopy
- Immunogold Techniques
- Monoclonal Antibodies