Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Scharnagl, H; März, W; Schliack, M; Löser, R; Gross, W.
A novel assay for cytosolic 3-hydroxy-3-methylglutaryl-coenzyme A synthase activity using reversed-phase ion-pair chromatography: demonstration that Lifibrol (K12.148) modulates the enzyme activity.
J LIPID RES 1995 36: 622-627.
Doi: 10.1016/S0022-2275(20)39896-5
[OPEN ACCESS]
Web of Science
PubMed
FullText
FullText_MUG
Google Scholar
- Führende Autor*innen der Med Uni Graz
- Scharnagl Hubert
- Co-Autor*innen der Med Uni Graz
- März Winfried
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- Cytosolic HMG-CoA synthase and microsomal 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) reductase catalyze two sequential steps in the mevalonate pathway. Both enzymes are negatively regulated by cholesterol. Cytosolic HMG-CoA synthase is responsible for the generation of HMG-CoA from acetyl-CoA and acetoacetyl-CoA). We have developed a new method to determine HMG-CoA synthase activity. In this assay, HMG-CoA is formed from acetoacetyl-CoA and labeled acetyl-CoA. The HMG-CoA product is isolated from the reaction mixture by means of reversed-phase ion-pair chromatography. The recovery of the product was always greater than 90%. The average within-batch coefficient of variation for HMG-CoA synthase activity was 5.1%. Using the new assay, we demonstrate that Lifibrol (K12.148), a new hypolipidemic compound, inhibits HMG-CoA synthase. Because our assay is accurate and precise it may become useful in future studies on the regulation and the pharmacological modulation of cytosolic HMG-CoA synthase.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Antilipemic Agents - pharmacology
- Butanols - pharmacology
- Chickens - pharmacology
- Chromatography, Ion Exchange - methods
- Cytosol - enzymology
- Hydroxybenzoic Acids - pharmacology
- Hydroxymethylglutaryl-CoA Synthase - analysis
- Liver - enzymology
- Research Support, Non-U.S. Gov't - enzymology
- Find related publications in this database (Keywords)
- MEVALONATE PATHWAY
- HMG-COA