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Hennerbichler, S; Méhes, G; Schmied, R; Wintersteiger, R; Dohr, G; Ambros, P; Sedlmayr, P.
Detection and relocation of rare events. A comparative study using the laser scanning cytometer and the Metafer/RCDetect microscope scanning system.
J Biochem Biophys Methods. 2002; 53(1-3):109-115 Doi: 10.1016%2FS0165-022X%2802%2900098-2
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Leading authors Med Uni Graz: Sedlmayr Peter
Co-authors Med Uni Graz: Dohr Gottfried
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Abstract:: We compared instrumental analysis of enriched cord blood nucleated red blood cells (CB-NRBC) out of in vitro contamination preparations of dilutions of minute volumes of male cord blood into peripheral blood from nonpregnant women. This was done using the laser scanning cytometer (LSC) and the Metafer/RCDetect microscope scanning system, both allowing for relocation of positive cells defined on the basis of fluorescence parameters. Both instruments were efficient in performing scanning and relocation; a difference in the recovery of CB-NRBC was not significant and can be explained by the method of preparation used.
Find related publications in this database (using NLM MeSH Indexing): Animals -; Cell Separation - instrumentation; Equipment Failure Analysis - instrumentation; Erythroblasts - classification; Female - classification; Fetal Blood - cytology; Flow Cytometry - instrumentation; Humans - instrumentation; Image Cytometry - instrumentation; In Situ Hybridization, Fluorescence - instrumentation; Infant, Newborn - instrumentation; Lasers - instrumentation; Male - instrumentation; Maternal-Fetal Exchange - instrumentation; Microscopy, Confocal - methods; Microscopy, Fluorescence - instrumentation; Pregnancy - instrumentation; Y Chromosome - instrumentation
Find related publications in this database (Keywords): laser scanning cytometry; Metafer/RCDetect; rare cells; magnetic cell sorting