Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Sedlmayr, P; Morales, P; Trummer, S; Wascher, K; Azzola, D; Blaschitz, A; Hammer, A; Walcher, W; Wintersteiger, R; Dohr, G.
Absence of HLA-G expression in macrophages of human decidua.
Am J Reprod Immunol. 2002; 48(2):96-102
Doi: 10.1034%2Fj.1600-0897.2002.01116.x
Web of Science
PubMed
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- Führende Autor*innen der Med Uni Graz
- Sedlmayr Peter
- Co-Autor*innen der Med Uni Graz
- Blaschitz Astrid
- Dohr Gottfried
- Hammer Astrid
- Walcher Wolfgang
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- Abstract:
- PROBLEM: Macrophages - together with natural killer (NK) cells - constitute the majority of bone marrow derived infiltrating cells in the decidua. As interferon-gamma (IFN-gamma), a cytokine produced by NK cells, has been reported to induce expression of human leukocyte antigen (HLA-G) in monocytic cells, suggesting expression of HLA-G on decidua macrophages potentially stimulated by IFN-gamma, the question arises whether decidua macrophages in normal pregnancy express HLA-G. METHOD OF STUDY: The study was based on immunohistochemistry and flow cytometry. In order to exclude that potentially elusive soluble HLA-G was not detected by immunohistochemistry, we performed in addition RT-PCR of flow-sorted decidua macrophages. RESULTS: Our findings indicate that HLA-G is not present on macrophages of first trimester or term decidua in either membrane-bound or soluble form. Transcripts for soluble HLA-G1 and -G2 were not detected. CONCLUSIONS: We exclude a role of HLA-G on the surface of decidua macrophages or of soluble HLA-G1 or -G2 as a secretory product of decidua macrophages with regard to interaction with HLA-G receptors present in or outside the decidua.
- Find related publications in this database (using NLM MeSH Indexing)
- Cell Line -
- Decidua - immunology
- Female - immunology
- Flow Cytometry - immunology
- Gene Expression - immunology
- HLA Antigens - immunology
- Histocompatibility Antigens Class I - immunology
- Humans - immunology
- Immunohistochemistry - immunology
- Macrophages - immunology
- Pregnancy - immunology
- Pregnancy Trimesters - immunology
- Reverse Transcriptase Polymerase Chain Reaction - immunology
- Transfection - immunology
- Find related publications in this database (Keywords)
- flow cytometry
- IFN-gamma
- immunohistochemistry
- MHC-1b
- placenta