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Selected Publication:
Hennerbichler, S; Schmied, R; Petek, E; Kroisel, PM; Pertl, B; Tiran, B; Dohr, G; Wintersteiger, R; Sedlmayr, P.
Detection and relocation of cord blood nucleated red blood cells by laser scanning cytometry.
Cytometry. 2002; 48(2):87-92
Doi: 10.1002/cyto.10112
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- Leading authors Med Uni Graz
- Sedlmayr Peter
- Co-authors Med Uni Graz
- Dohr Gottfried
- Kroisel Peter
- Pertl Barbara
- Petek Erwin
- Tiran Beate
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- Abstract:
- BACKGROUND: Fetal nucleated red blood cells (NRBC) present in the peripheral blood of pregnant women at low frequency are a potential target for noninvasive prenatal diagnostics. METHODS: CD71-enriched cells from male cord blood (CB) were stained for the gamma chain of HbF (Hb-gamma) and cytocentrifuged. Fluorescence in situ hybridization (FISH) was done for the Y chromosome. Following staining of the nucleus with TO-PRO-3, laser scanning cytometry was performed. Artificial mixtures of small volumes of male CB and blood drawn from nonpregnant females were analyzed. RESULTS: In CB, 59% of events double positive for Hb-gamma and TO-PRO-3 were identified as CB-NRBC. In contamination studies, male fetal CB-NRBC were identified perfectly on the basis of morphologic characteristics and FISH reactivity following relocation and visual assessment. Mean recovery was 8.7%. CONCLUSIONS: Laser scanning cytometry of preenriched fetal NRBC may offer a promising way for noninvasive prenatal diagnostics. This is because it provides a virtual enrichment step and the position on the slides of cells visually confirmed to correspond to fetal NRBC is known. Further experimental procedures on well-defined and located target cells may be feasible.
- Find related publications in this database (using NLM MeSH Indexing)
- Cell Nucleus - metabolism
- Chromosomes, Human, Y - metabolism
- Erythroblasts - cytology
- Female - cytology
- Fetal Blood - cytology
- Humans - cytology
- Image Cytometry - methods
- In Situ Hybridization, Fluorescence - methods
- Infant, Newborn - methods
- Male - methods
- Microscopy, Confocal - methods
- Prenatal Diagnosis - methods
- Staining and Labeling - methods
- Find related publications in this database (Keywords)
- LSC
- erythroblasts
- prenatal diagnostics