Medizinische Universität Graz - Research portal
Selected Publication:
Bowles, DK; Graier, WF; Sturek, M.
Hydrogen peroxide activates Na(+)-dependent Ca(2+) influx in coronary endothelial cells.
Biochem Biophys Res Commun. 2001; 287(5):1134-1139
Doi: 10.1006/bbrc.2001.5714
Web of Science
PubMed
FullText
FullText_MUG
- Co-authors Med Uni Graz
- Graier Wolfgang
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- The purpose of the present study was to examine the effect of short duration H(2)O(2) exposure on coronary artery endothelial cell [Ca(2+)](i) regulation. Freshly dispersed cells from porcine coronary artery were exposed to H(2)O(2) (300 micromol/L) for 3 min while monitoring [Ca(2+)](i) using fura-2 microfluorometry. H(2)O(2) increased [Ca(2+)](i) from 0.86 +/- 0.03 to 2.19 +/- 0.41 ratio units at 3 min of H(2)O(2) (P < 0.05). Intracellular Ca(2+) remained elevated 3 min following removal of H(2)O(2), yet H(2)O(2) had no effect on the subsequent [Ca(2+)](i) response to bradykinin (0.1 micromol/L). The H(2)O(2)-induced [Ca(2+)](i) increase was completely abolished either by removal of extracellular Ca(2+) or lowering extracellular Na(+). Cells exposed to the Na(+) ionophore, monensin, showed an increase in [Ca(2+)](i) with a time course similar to that seen with H(2)O(2). Furthermore, H(2)O(2)-induced Ca(2+) influx was not attenuated by either Ni(2+) (300 micromol/L) or econazole (10 micromol/L), excluding Ca(2+) influx via the agonist-sensitive pathway. Thus, in coronary arterial endothelial cells, H(2)O(2) increases Ca(2+) influx in an extracellular Na(+)-dependent manner via an agonist-insensitive pathway.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Arteries - metabolism
- Biological Transport - metabolism
- Bradykinin - pharmacology
- Calcium - metabolism
- Cations - metabolism
- Cattle - metabolism
- Coronary Circulation - physiology
- Endothelium, Vascular - metabolism
- Hydrogen Peroxide - pharmacology
- Ionophores - pharmacology
- Monensin - pharmacology
- Oxidative Stress - physiology
- Sodium - metabolism
- Find related publications in this database (Keywords)
- hydrogen peroxide
- oxidative stress
- endothelium
- intracellular Ca2+
- Ca2+ influx
- porcine