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Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Schmaler, M; Colone, A; Spagnuolo, J; Zimmermann, M; Lepore, M; Kalinichenko, A; Bhatia, S; Cottier, F; Rutishauser, T; Pavelka, N; Egli, A; Azzali, E; Pieroni, M; Costantino, G; Hruz, P; Sauer, U; Mori, L; De, Libero, G.
Modulation of bacterial metabolism by the microenvironment controls MAIT cell stimulation.
Mucosal Immunol. 2018; 11(4): 1060-1070.
Doi: 10.1038/s41385-018-0020-9
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- Co-authors Med Uni Graz
- Kalinichenko Artem
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- Abstract:
- Mucosal-associated invariant T (MAIT) cells are abundant innate-like T lymphocytes in mucosal tissues and recognize a variety of riboflavin-related metabolites produced by the microbial flora. Relevant issues are whether MAIT cells are heterogeneous in the colon, and whether the local environment influences microbial metabolism thereby shaping MAIT cell phenotypes and responses. We found discrete MAIT cell populations in human colon, characterized by the diverse expression of transcription factors, cytokines and surface markers, indicative of activated and precisely controlled lymphocyte populations. Similar phenotypes were rare among circulating MAIT cells and appeared when circulating MAIT cells were stimulated with the synthetic antigens 5-(2-oxoethylideneamino)-6-D-ribitylaminouracil, and 5-(2-oxopropylideneamino)-6-D-ribitylaminouracil. Furthermore, bacteria grown in colon-resembling conditions with low oxygen tension and harvested at stationary growth phase, potently activated human MAIT cells. The increased activation correlated with accumulation of the above antigenic metabolites as indicated by mass spectrometry. Thus, the colon environment contributes to mucosal immunity by directly affecting bacterial metabolism, and indirectly controlling the stimulation and differentiation of MAIT cells.
- Find related publications in this database (using NLM MeSH Indexing)
- Antigens, Bacterial - immunology
- Cell Differentiation - administration & dosage
- Cells, Cultured - administration & dosage
- Cellular Microenvironment - administration & dosage
- Colon - pathology
- Gastrointestinal Microbiome - physiology
- Humans - administration & dosage
- Immunity, Innate - administration & dosage
- Immunization - administration & dosage
- Mucosal-Associated Invariant T Cells - immunology
- Riboflavin - immunology
- Uracil - analogs & derivatives, immunology