Medizinische Universität Graz - Research portal

Go directly to the nagivation.
Go directly to the content.

Navigation/Search

Selected Publication:

SHR Neuro Cancer Cardio Lipid Metab Microb

German, Y; Vulliard, L; Kamnev, A; Pfajfer, L; Huemer, J; Mautner, AK; Rubio, A; Kalinichenko, A; Boztug, K; Ferrand, A; Menche, J; Dupré, L.
Morphological profiling of human T and NK lymphocytes by high-content cell imaging.
Cell Rep. 2021; 36(1): 109318 Doi: 10.1016/j.celrep.2021.109318
PubMed FullText FullText_MUG

Co-authors Med Uni Graz: Kalinichenko Artem
Altmetrics:
Dimensions Citations:
Plum Analytics:
Scite (citation analytics):
Abstract:: The immunological synapse is a complex structure that decodes stimulatory signals into adapted lymphocyte responses. It is a unique window to monitor lymphocyte activity because of development of systematic quantitative approaches. Here we demonstrate the applicability of high-content imaging to human T and natural killer (NK) cells and develop a pipeline for unbiased analysis of high-definition morphological profiles. Our approach reveals how distinct facets of actin cytoskeleton remodeling shape immunological synapse architecture and affect lytic granule positioning. Morphological profiling of CD8⁺ T cells from immunodeficient individuals allows discrimination of the roles of the ARP2/3 subunit ARPC1B and the ARP2/3 activator Wiskott-Aldrich syndrome protein (WASP) in immunological synapse assembly. Single-cell analysis further identifies uncoupling of lytic granules and F-actin radial distribution in ARPC1B-deficient lymphocytes. Our study provides a foundation for development of morphological profiling as a scalable approach to monitor primary lymphocyte responsiveness and to identify complex aspects of lymphocyte micro-architecture.
Find related publications in this database (using NLM MeSH Indexing): Actin-Related Protein 2-3 Complex - deficiency, metabolism; Adolescent - administration & dosage; CD8-Positive T-Lymphocytes - cytology, drug effects; Cell Line - administration & dosage; Cell Shape - drug effects; Cytoskeleton - drug effects, metabolism; Exocytosis - drug effects; Humans - administration & dosage; Imaging, Three-Dimensional - administration & dosage; Immunological Synapses - drug effects, metabolism; Killer Cells, Natural - cytology, drug effects, metabolism; Male - administration & dosage; Organoselenium Compounds - pharmacology; Organosilicon Compounds - pharmacology; Single-Cell Analysis - administration & dosage; T-Lymphocytes - cytology, drug effects, metabolism; Thiones - pharmacology; Uracil - analogs & derivatives, pharmacology; Wiskott-Aldrich Syndrome Protein - deficiency, metabolism