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SHR Neuro Cancer Cardio Lipid Metab Microb

Bernecker, C; Rosskopf, K; Url, C; Raicht, A; Schlenke, P.
CD34 Positive Selection of Cryopreserved Stem Cell Concentrates with the CliniMACS Prodigy Platform and the Tubing Set TS 320: Preclinical Results from a Validation Study
TRANSFUS MED HEMOTH. 2025; Doi: 10.1159/000549364 [OPEN ACCESS]
Web of Science PubMed FullText FullText_MUG

Leading authors Med Uni Graz: Bernecker Claudia; Rosskopf Konrad
Co-authors Med Uni Graz: Raicht Andrea; Schlenke Peter
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Abstract:: INTRODUCTION: Immuno-magnetic CD34 positive (CD34+) selection is generally used for stem cell boosts after allogeneic stem cell transplantation. In some cases, only cryopreserved cells are available as starting material. We present a new automatic device and technique for preparing and subsequently magnetically enriching these cells. METHODS: We used the CliniMACS Prodigy® platform in combination with the TS 320 tubing set, equipped with a large 800 mL chamber, which enables to include the DMSO wash, platelet wash, antibody incubation, and antibody wash and the subsequent CD34 enrichment on an automatic basis with only short hands-on time. We performed three validation runs using DNase and sodium citrate for cell preparation to reduce the risk of aggregates and clots. RESULTS: Our robust preclinical results show the feasibility and safety of the process with a mean of 53% CD34 yield after thawing and CD34 purity and viability of 93 and 97%, respectively. CD3 log depletion exceeds 5.0 in all 3 cases, which provides optimal GvHD prevention. CONCLUSION: This shows that the updated technique gains unaffected CD34+ cells of high quality, not only from fresh but also from cryopreserved stem cell products.
Find related publications in this database (Keywords): Immuno-magnetic CD34+selection; Cryopreserved stem cell concentrates; CliniMACS prodigy; Stem cell boost