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SHR Neuro Cancer Cardio Lipid Metab Microb
Weinberger, V; Mohammadzadeh, R; Blohs, M; Kalt, K; Mahnert, A; Moser, S; Cecovini, M; Mertelj, P; Zurabishvili, T; Arora, B; Wolf, J; Shinde, T; Madl, T; Habisch, H; Kolb, D; Pernitsch, D; Hingerl, K; Metcalf, W; Moissl-Eichinger, C.
Expanding the cultivable human archaeome: Methanobrevibacter intestini sp. nov. and strain Methanobrevibacter smithii 'GRAZ-2' from human faeces
INT J SYST EVOL MICR. 2025; 75(4): 006751
Doi: 10.1099/ijsem.0.006751
Web of Science
PubMed
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- Leading authors Med Uni Graz
- Moissl-Eichinger Christine
- Weinberger Viktoria
- Co-authors Med Uni Graz
- Arora Bhawna
- Blohs Marcus
- Habisch Hansjörg
- Hingerl Kerstin
- Kolb Dagmar
- Madl Tobias
- Mahnert Alexander
- Mohammadzadeh Rokhsareh
- Pernitsch Dominique
- Shinde Tejus
- Zurabishvili Tamara
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- Abstract:
- Two mesophilic, hydrogenotrophic methanogens, WWM1085 and M. smithii GRAZ-2, were isolated from human faecal samples. WWM1085 was isolated from an individual in the United States and represents a novel species within the genus Methanobrevibacter. M. smithii GRAZ-2 (=DSM 116045) was retrieved from a faecal sample of a European, healthy woman and represents a novel strain within this species. Both Methanobrevibacter representatives form non-flagellated, short rods with variable morphologies and the capacity to form filaments. Both isolates showed the typical fluorescence of F420 and methane production. Compared to M. smithii GRAZ-2, WWM1085 did not accumulate formate when grown with H2 and CO2. The optimal growth conditions were at 35-39 degrees C and pH 6.5-7.5. Full genome sequencing revealed a genomic difference of WWM1085 to the type strain of M. smithii DSM 861 (=PST), with 93.55% average nucleotide identity (ANI) and major differences in the sequence of its mcrA gene (3.3% difference in nucleotide sequence). Differences in the 16S rRNA gene sequence were very minor, and thus distinction based on this gene marker might not be possible. M. smithii GRAZ-2 was identified as a novel strain within the species Methanobrevibacter smithii (ANI 99.04% to M. smithii DSM 861 [=PST]). Due to the major differences between WWM1085 and M. smithiitype strain M. smithii DSM 861 (=PST) in phenotypic, genomic and metabolic features, we propose Methanobrevibacter intestini sp. nov. as a novel species with WWM1085 as the type strain (DSM 116060T = CECT 30992T).
- Find related publications in this database (Keywords)
- faecal methanogens
- Methanobrevibacter intestini
- Methanobrevibacter smithii
- human archaeome.