Medizinische Universität Graz - Research portal

Go directly to the nagivation.
Go directly to the content.

Navigation/Search

Selected Publication:

SHR Neuro Cancer Cardio Lipid Metab Microb

Hirtl, M; Gottschalk, B; Bachkoenig, OA; Oflaz, FE; Madreiter-Sokolowski, C; Høydal, MA; Graier, WF.
A novel super-resolution STED microscopy analysis approach to observe spatial MCU and MICU1 distribution dynamics in cells.
Biochim Biophys Acta Mol Cell Res. 2025; 1872(3):119900 Doi: 10.1016/j.bbamcr.2025.119900
Web of Science PubMed FullText FullText_MUG

Leading authors Med Uni Graz: Graier Wolfgang; Hirtl Martin
Co-authors Med Uni Graz: Bachkönig Olaf Arne Georg; Gottschalk Benjamin; Madreiter-Sokolowski Corina; Oflaz Furkan Enes
Altmetrics:
Dimensions Citations:
Plum Analytics:
Scite (citation analytics):
Abstract:: The uptake of Ca²⁺ by mitochondria is an important and tightly controlled process in various tissues. Even small changes in the key proteins involved in this process can lead to significant cellular dysfunction and, ultimately, cell death. In this study, we used stimulated emission depletion (STED) microscopy and developed an unbiased approach to monitor the sub-mitochondrial distribution and dynamics of the mitochondrial calcium uniporter (MCU) and mitochondrial calcium uptake 1 (MICU1) under resting and stimulated conditions. To visualize the inner mitochondrial membrane, the STED-optimized dye called pkMitoRed was used. The study presented herein builds on the previously verified exclusive localization of MICU1 in the intermembrane space, and that MCU moves exclusively laterally along the inner mitochondrial membrane (IMM). We applied a multi-angled arrow histogram to analyze the distribution of proteins within mitochondria, providing a one-dimensional view of protein localization along a defined distance. Combining this with optimal transport colocalization enabled us to further predict submitochondrial protein distribution. Results indicate that in HeLa cells Ca²⁺ elevation yielded MCU translocation from the cristae membrane (CM) to the inner boundary membrane (IBM). In AC16 cardiomyocyte cell line, MCU is mainly located at the IBM under resting conditions, and it translocates to the CM upon rising Ca²⁺. Our data describe a novel unbiased super-resolution image analysis approach. Our showcase sheds light on differences in spatial distribution dynamics of MCU in cell lines with different MICU1:MCU abundance.
Find related publications in this database (using NLM MeSH Indexing): Humans - administration & dosage; HeLa Cells - administration & dosage; Mitochondrial Membrane Transport Proteins - metabolism, genetics; Calcium Channels - metabolism, genetics; Mitochondria - metabolism; Calcium-Binding Proteins - metabolism, genetics; Mitochondrial Membranes - metabolism; Cation Transport Proteins - metabolism, genetics; Calcium - metabolism; Protein Transport - administration & dosage; Microscopy, Fluorescence - methods
Find related publications in this database (Keywords): Mitochondrial calcium uniporter (MCU); Mitochondrial calcium uptake 1 (MICU1); Stimulated-emission depletion (STED); Inner mitochondrial membrane (IMM); Structured illumination microscopy (SIM)