Medizinische Universität Graz - Research portal
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SHR Neuro Cancer Cardio Lipid Metab Microb
Pomastowski, P; Sprynskyy, M; Žuvela, P; Rafińska, K; Milanowski, M; Liu, JJ; Yi, M; Buszewski, B.
Silver-Lactoferrin Nanocomplexes as a Potent Antimicrobial Agent.
J Am Chem Soc. 2016; 138(25): 7899-909.
Doi: 10.1021/jacs.6b02699
Web of Science
PubMed
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- Co-authors Med Uni Graz
- Monedeiro-Milanowski Maciej
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- Abstract:
- The process of silver immobilization onto and/or into bovine lactoferrin (LTF), the physicochemical properties of bovine lactoferrin and obtained silver-lactoferrin complexes, as well as antibacterial activity of silver-lactoferrin complexes were investigated in this work. Kinetic study of the silver immobilization into lactoferrin was carried out using batch sorption techniques. Spectrometric (MALDI-TOF/TOF-MS, ICP-MS), spectroscopic (FTIR, SERS), electron microscopic (TEM) and electrophoretic (I-DE) techniques, as well as zeta potential measurements, were applied for characterization of LTF and binding nature of silver in Ag-LTF complexes. On the basis of the results of the kinetics study, it was established that the silver binding to LTF is a heterogeneous process involving two main stages: (i) internal diffusion and sorption onto external surface of lactoferrin globules; and (ii) internal diffusion and binding into lactoferrin globule structure. Spectroscopic techniques combined with TEM analysis confirmed the binding process. Molecular dynamics (MD) analysis was carried out in order to simulate the mechanism of the binding process, and locate potential binding sites, as well as complement the experimental findings. Quantum mechanics (QM) simulations were performed utilizing density functional theory (DFT) in order to support the reduction mechanism of silver ions to elemental silver. Antimicrobial activity of synthesized lactoferrin complexes against selected clinical bacteria was confirmed using flow cytometry and antibiograms.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals - administration & dosage
- Anti-Infective Agents - chemistry
- Aspartic Acid - chemistry
- Bacteria - drug effects
- Binding Sites - administration & dosage
- Cattle - administration & dosage
- Drug Design - administration & dosage
- Glutamic Acid - chemistry
- Ions - administration & dosage
- Lactoferrin - chemistry
- Metal Nanoparticles - chemistry
- Microbial Sensitivity Tests - administration & dosage
- Molecular Dynamics Simulation - administration & dosage
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- Protein Binding - administration & dosage
- Pseudomonas aeruginosa - drug effects
- Quantum Theory - administration & dosage
- Silver - chemistry
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- Surface Properties - administration & dosage