Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Niehaus, P; Gonzalez, de, Vega, R; Haindl, MT; Birkl, C; Leoni, M; Birkl-Toeglhofer, AM; Haybaeck, J; Ropele, S; Seeba, M; Goessler, W; Karst, U; Langkammer, C; Clases, D.
Multimodal analytical tools for the molecular and elemental characterisation of lesions in brain tissue of multiple sclerosis patients.
Talanta. 2024; 270:125518
Doi: 10.1016/j.talanta.2023.125518
Web of Science
PubMed
FullText
FullText_MUG
- Leading authors Med Uni Graz
- Langkammer Christian
- Co-authors Med Uni Graz
- Birkl Christoph
- Birkl-Töglhofer Anna Maria
- Haindl Michaela Tanja
- Haybäck Johannes
- Leoni Marlene
- Ropele Stefan
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- Abstract:
- Multiple sclerosis (MS) is a prevalent immune-mediated inflammatory disease of the central nervous system inducing a widespread degradation of myelin and resulting in neurological deficits. Recent advances in molecular and atomic imaging provide the means to probe the microenvironment in affected brain tissues at an unprecedented level of detail and may provide new insights. This study showcases state-of-the-art spectroscopic and mass spectrometric techniques to compare distributions of molecular and atomic entities in MS lesions and surrounding brain tissues. MS brains underwent post-mortem magnetic resonance imaging (MRI) to locate and subsequently dissect MS lesions and surrounding white matter. Digests of lesions and unaffected white matter were analysed via ICP-MS/MS revealing significant differences in concentrations of Li, Mg, P, K, Mn, V, Rb, Ag, Gd and Bi. Micro x-ray fluorescence spectroscopy (μXRF) and laser ablation - inductively coupled plasma - time of flight - mass spectrometry (LA-ICP-ToF-MS) were used as micro-analytical imaging techniques to study distributions of both endogenous and xenobiotic elements. The essential trace elements Fe, Cu and Zn were subsequently calibrated using in-house manufactured gelatine standards. Lipid distributions were studied using IR-micro spectroscopy and matrix assisted laser desorption/ionisation mass spectrometry imaging (MALDI-MSI). MALDI-MSI was complemented with high-resolution tandem mass spectrometry and trapped ion mobility spectroscopy for the annotation of specified phospho- and sphingolipids, revealing specific lipid species decreased in MS lesions compared to surrounding white matter. This explorative study demonstrated that modern molecular and atomic mapping techniques provide high-resolution imaging for relevant bio-indicative entities which may complement our current understanding of the underlying pathophysiological processes.
- Find related publications in this database (using NLM MeSH Indexing)
- Humans - administration & dosage
- Multiple Sclerosis - diagnostic imaging, pathology
- Tandem Mass Spectrometry - administration & dosage
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
- Brain - diagnostic imaging
- Lipids - administration & dosage
- Find related publications in this database (Keywords)
- MALDI-MSI
- XRF
- LA-ICP-MS
- TOF
- IR imaging