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SHR Neuro Cancer Cardio Lipid Metab Microb
Amor, M; Bianco, V; Buerger, M; Lechleitner, M; Vujić, N; Dobrijević, A; Akhmetshina, A; Pirchheim, A; Schwarz, B; Pessentheiner, AR; Baumgartner, F; Rampitsch, K; Schauer, S; Klobučar, I; Degoricija, V; Pregartner, G; Kummer, D; Svecla, M; Sommer, G; Kolb, D; Holzapfel, GA; Hoefler, G; Frank, S; Norata, GD; Kratky, D.
Genetic deletion of MMP12 ameliorates cardiometabolic disease by improving insulin sensitivity, systemic inflammation, and atherosclerotic features in mice.
Cardiovasc Diabetol. 2023; 22(1): 327
Doi: 10.1186/s12933-023-02064-3
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- Leading authors Med Uni Graz
- Amor Melina
- Kratky Dagmar
- Co-authors Med Uni Graz
- Akhmetshina Alena
- Bianco Valentina
- Bürger Martin
- Frank Sasa
- Höfler Gerald
- Kolb Dagmar
- Kummer Daniel
- Lechleitner Margarete
- Pessentheiner Ariane Raphaela
- Pirchheim Anita
- Pregartner Gudrun
- Schauer Silvia
- Sommer Gerhard
- Vujic Nemanja
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- Abstract:
- BACKGROUND: Matrix metalloproteinase 12 (MMP12) is a macrophage-secreted protein that is massively upregulated as a pro-inflammatory factor in metabolic and vascular tissues of mice and humans suffering from cardiometabolic diseases (CMDs). However, the molecular mechanisms explaining the contributions of MMP12 to CMDs are still unclear. METHODS: We investigated the impact of MMP12 deficiency on CMDs in a mouse model that mimics human disease by simultaneously developing adipose tissue inflammation, insulin resistance, and atherosclerosis. To this end, we generated and characterized low-density lipoprotein receptor (Ldlr)/Mmp12-double knockout (DKO) mice fed a high-fat sucrose- and cholesterol-enriched diet for 16-20 weeks. RESULTS: DKO mice showed lower cholesterol and plasma glucose concentrations and improved insulin sensitivity compared with LdlrKO mice. Untargeted proteomic analyses of epididymal white adipose tissue revealed that inflammation- and fibrosis-related pathways were downregulated in DKO mice. In addition, genetic deletion of MMP12 led to alterations in immune cell composition and a reduction in plasma monocyte chemoattractant protein-1 in peripheral blood which indicated decreased low-grade systemic inflammation. Aortic en face analyses and staining of aortic valve sections demonstrated reduced atherosclerotic plaque size and collagen content, which was paralleled by an improved relaxation pattern and endothelial function of the aortic rings and more elastic aortic sections in DKO compared to LdlrKO mice. Shotgun proteomics revealed upregulation of anti-inflammatory and atheroprotective markers in the aortas of DKO mice, further supporting our data. In humans, MMP12 serum concentrations were only weakly associated with clinical and laboratory indicators of CMDs. CONCLUSION: We conclude that the genetic deletion of MMP12 ameliorates obesity-induced low-grade inflammation, white adipose tissue dysfunction, biomechanical properties of the aorta, and the development of atherosclerosis. Therefore, therapeutic strategies targeting MMP12 may represent a promising approach to combat CMDs.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals - administration & dosage
- Humans - administration & dosage
- Mice - administration & dosage
- Atherosclerosis - genetics, prevention & control
- Cholesterol - administration & dosage
- Disease Models, Animal - administration & dosage
- Inflammation - genetics, metabolism
- Insulin Resistance - administration & dosage
- Matrix Metalloproteinase 12 - genetics
- Mice, Inbred C57BL - administration & dosage
- Mice, Knockout - administration & dosage
- Plaque, Atherosclerotic - administration & dosage
- Proteomics - administration & dosage
- Receptors, LDL - genetics
- Find related publications in this database (Keywords)
- CMD
- Matrix metalloproteinase 12
- MMP12 deficiency
- Ldlr-deficient mice
- Proteomics
- Metabolic syndrome patients