Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Robinson, I; Bertsch, A; Leithner, K; Stiegler, P; Olschewski, H; Hrzenjak, A.
Circulating microRNAs as molecular biomarkers for lung adenocarcinoma.
Cancer Biomark. 2022; 34(4):591-606
Doi: 10.3233/CBM-210205
Web of Science
PubMed
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FullText_MUG
- Leading authors Med Uni Graz
- Hrzenjak Andelko
- Co-authors Med Uni Graz
- Birnstingl Alexandra
- Leithner Katharina
- Olschewski Horst
- Stiegler Philipp
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- Abstract:
- BACKGROUND: The potential of microRNAs (miRNAs) as molecular tumor biomarkers for early diagnosis and prognosis in lung cancer is still unclear. OBJECTIVE: To analyze expression of miRNAs in A549 lung adenocarcinoma (LUAD) cells and in primary, non-malignant bronchial epithelial (BE) cells from healthy donors. To analyze the most prominently deregulated miRNAs in plasma samples of LUAD patients and healthy donors. MATERIALS AND METHODS: The expression of 752 miRNAs in LUAD and BE cells was assessed by RT-qPCR with mean-centering restricted normalization. The relative plasma levels of 18 miRNAs in LUAD patients and healthy donors were analyzed using RT-qPCR and normalized to miR-191-5p and miR-16-3p. Putative interactions between miRNAs and their target genes were investigated in silico. RESULTS: Out of 752 miRNAs, 37 miRNAs were significantly deregulated in A549 cells compared to BE cells. MiR-15b-3p, miR-148a-3p, miR-193b-3p, and miR-195-5p were significantly deregulated in plasma samples of LUAD patients compared to donors. The target genes of those four miRNAs are involved in essential mechanisms in cancer development and progression. CONCLUSIONS: There are substantial differences between cancer and control miRNA expression in vitro and in plasma samples of LUAD patients compared to healthy donors. Four deregulated miRNAs are promising as a diagnostic biomarker for adenocarcinoma of the lung.
- Find related publications in this database (using NLM MeSH Indexing)
- Adenocarcinoma of Lung - pathology
- Biomarkers, Tumor - genetics
- Circulating MicroRNA - administration & dosage
- Gene Expression Regulation, Neoplastic - administration & dosage
- Humans - administration & dosage
- Lung Neoplasms - diagnosis, genetics
- MicroRNAs - metabolism
- Find related publications in this database (Keywords)
- Lung adenocarcinoma
- A549 cells
- bronchial epithelial cells
- microRNA
- plasma samples