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Selected Publication:
Cervar, M; Blaschitz, A; Dohr, G; Desoye, G.
Paracrine regulation of distinct trophoblast functions in vitro by placental macrophages.
Cell Tissue Res. 1999; 295(2):297-305
Doi: 10.1007%2Fs004410051236
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- Leading authors Med Uni Graz
- Cervar-Zivkovic Mila
- Desoye Gernot
- Co-authors Med Uni Graz
- Blaschitz Astrid
- Dohr Gottfried
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- Abstract:
- In view of the accumulating evidence for paracrine mechanisms regulating trophoblast function, we tested the hypothesis that placental macrophages affect trophoblast activity in a paracrine fashion. Trophoblast was isolated from 17 term placentas (-IP). One aliquot of cells was further immunopurified (+IP) using an HLA class I antibody. This increased the proportion of trophoblast (+IP >97%; -IP approximately 70%) as identified by rigorous immunocytochemistry. Most (approximately 70%) non-trophoblast cells in -IP were macrophages. The cells were cultured for 5 days with a daily medium change. In addition, +IP cells from seven placentas were cultured with lipopolysaccharide (LPS)-stimulated or -unstimulated macrophage-conditioned media. The concentrations of lactate, trophoblast-specific hormones, human chorionic gonadotropin-beta (hCG-beta) and human placental lactogen (hPL), of several prostanoids and of endothelin-1 and angiotensin II were determined in the culture media. The accumulated amounts of substances released into the culture media, corrected for the greater proportion of trophoblast in +IP cultures, were on average two- to threefold higher (hCG-beta: 18-fold) in +IP than in -IP, with the exception of endothelin-1,2 (no change), angiotensin II (-70%) and 6-keto-prostaglandin-F1alpha (-40%). [3H]leucine incorporation into the trichloroacetic acid (TCA)-precipitable pool measured on day 5 was twofold higher in +IP than in -IP. Addition of conditioned media reverted these changes. The data demonstrate that placental macrophages in culture affect trophoblast biosynthetic activity in a paracrine fashion. We conclude that macrophages are important regulators of trophoblast activity.
- Find related publications in this database (using NLM MeSH Indexing)
- Cell Communication - physiology
- Cells, Cultured - physiology
- Coculture Techniques - physiology
- Female - physiology
- Humans - physiology
- Macrophages - cytology
- Paracrine Communication - physiology
- Placenta - cytology
- Trophoblasts - cytology
- Find related publications in this database (Keywords)
- Trophoblast
- Macrophages
- Placenta
- Cell Culture
- Paracrine Regulation
- Human