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SHR Neuro Cancer Cardio Lipid Metab Microb

Fluhr, H; Bischof-Islami, D; Krenzer, S; Licht, P; Bischof, P; Zygmunt, M.
Human chorionic gonadotropin stimulates matrix metalloproteinases-2 and -9 in cytotrophoblastic cells and decreases tissue inhibitor of metalloproteinases-1, -2, and -3 in decidualized endometrial stromal cells.
Fertil Steril. 2008; 90(4 Suppl):1390-5 Doi: 10.1016/j.fertnstert.2007.08.023
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Fluhr Herbert
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Abstract:
OBJECTIVE: To investigate the influence of hCG on trophoblastic matrix metalloproteinases (MMPs) -2 and -9 as well as endometrial tissue inhibitor of metalloproteinases (TIMPs) -1, -2, and -3. DESIGN: In vitro experiment. SETTING: Research laboratory at a university medical center. PATIENT(S): Women undergoing legal abortions and premenopausal women undergoing hysterectomy for benign reasons. INTERVENTION(S): Human first trimester cytotrophoblasts and decidualized endometrial stromal cells were incubated with recombinant hCG. MAIN OUTCOME MEASURE(S): Trophoblastic MMP-2 and -9 were analyzed by enzyme-linked immunosorbent assay (ELISA) and zymography, and endometrial TIMP-1, -2, and -3 were measured by real time reverse transcriptase-polymerase chain reaction and ELISA. RESULT(S): HCG increases the secretion of MMP-2 and -9 in cytotrophoblasts dose dependently. This effect occurs after 4 hours of incubation and becomes less pronounced after 24 hours. In contrast, TIMP-1, -2, and -3 are significantly reduced by hCG in endometrial stromal cells in a time- and dose-dependent manner. CONCLUSION(S): These results suggest a regulatory role of hCG on the MMP/TIMP system at the implantation site. By increasing trophoblastic MMP secretion and reducing endometrial TIMP expression, hCG may be an important tool for the invading embryo to regulate the depth of its implantation.
Find related publications in this database (using NLM MeSH Indexing)
Cells, Cultured - administration & dosage
Chorionic Gonadotropin - genetics, metabolism
Decidua - metabolism
Endometrium - metabolism
Female - administration & dosage
Gene Expression - administration & dosage
Humans - administration & dosage
Matrix Metalloproteinase 2 - genetics, metabolism
Matrix Metalloproteinase 9 - genetics, metabolism
Stromal Cells - metabolism
Tissue Inhibitor of Metalloproteinases - genetics, metabolism
Trophoblasts - metabolism

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