Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Fluhr, H; Krenzer, S; Zygmunt, M.
Different regulation of tissue inhibitors of metalloproteinases-1, -2 and -3 in human endometrial stromal cells during decidualization in vitro.
Reprod Med Biol. 2008; 7(4):169-175
Doi: 10.1111/j.1447-0578.2008.00213.x
[OPEN ACCESS]
PubMed
FullText
FullText_MUG
- Leading authors Med Uni Graz
- Fluhr Herbert
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- Aim: Endometrial tissue inhibitors of metalloproteinases (TIMPs) appear to play an essential role during early implantation by modulating the invasiveness of the trophoblast. The expression of TIMP-1, TIMP-2 and TIMP-3 in human endometrial stromal cells (ESC) was investigated during decidualization in vitro. Methods: Endometrial stromal cells were isolated from hysterectomy specimens from premenopausal women undergoing surgery for benign reasons. Decidualization in vitro was induced by the application of 1 µmol/L progesterone and 30 nmol/L 17β-estradiol over 9 days. The expression of TIMP-1, TIMP-2 and TIMP-3 in ESC was measured by semiquantitative real-time reverse transcription polymerase chain reaction and enzyme-linked immunosorbent assay over intervals of 3 days. Results: Decidualization in vitro was confirmed by a significant increase in prolactin expression. TIMP-1 and TIMP-2 mRNA and secreted protein showed no significant changes over the time-course of decidualization. In contrast, TIMP-3 was upregulated during the first 3 days of decidualization. An eightfold upregulation was observed until day 6, and the effect was less pronounced by day 9. Conclusion: These results suggest a regulatory role of the TIMP system for endometrial differentiation in the second half of the menstrual cycle and in early implantation. The expression pattern of endometrial TIMP-3 might be important for the regulation of trophoblast invasion. (Reprod Med Biol 2008; 7: 169-175).