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Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Spratte, J; Bornkessel, F; Schutz, F; Zygmunt, M; Fluhr, H.
The presence of heparins during decidualization modulates the response of human endometrial stromal cells to IL-1 beta in vitro
J ASSIST REPROD GEN. 2016; 33(7): 949-957.
Doi: 10.1007/s10815-016-0703-7
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- Co-authors Med Uni Graz
- Fluhr Herbert
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- Abstract:
- The aim of this paper is to study the impact of heparin on the response of human endometrial stromal cells (ESCs) to interleukin (IL)-1 beta during decidualization in vitro. ESCs were isolated from hysterectomy specimens of premenopausal women undergoing hysterectomy for benign reasons; decidualized in vitro and incubated in parallel with unfractionated heparin or tinzaparin; and stimulated with IL-1 beta at days 0, 3, 6, and 9 during decidualization. IL-6, IL-11, and leukemia inhibitory factor (LIF) were analyzed using ELISAs and real-time RT-PCR. Cell viability was determined by a fluorometric assay. IL-1 beta dose-dependently stimulated IL-6, IL-11, and LIF in distinct patterns in ESCs during decidualization. Unfractionated heparin as well as tinzaparin attenuated the IL-1 beta-mediated induction of IL-6, IL-11, and LIF on protein and messenger RNA (mRNA) levels. The relative effects of heparin and tinzaparin were getting more pronounced during the time course of decidualization. Unfractionated heparin and the low molecular weight heparin tinzaparin have modulating effects on IL-1 beta-induced endometrial cytokines of the IL-6 family during decidualization. These effects of heparins beyond their classical anti-coagulatory properties might have implications on the regulation of endometrial receptivity and early implantation.
- Find related publications in this database (Keywords)
- IL-1 beta
- Cytokines
- Decidualization
- Heparin