Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Zalyte, E; Dedonyte, V; Kurlinkus, B; Sileikis, A; Schemmer, P; Valius, M.
Establishment and Characterization of a New Pancreatic Ductal Adenocarcinoma Cell Line Capan-26.
ANTICANCER RES. 2021; 41(3): 1401-1406.
Doi: 10.21873/anticanres.14897
Web of Science
PubMed
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- Co-authors Med Uni Graz
- Schemmer Peter
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- Abstract:
- Pancreatic ductal adenocarcinoma is one of the deadliest forms of human cancer. Since only a vast panel of cell lines can fully recapitulate disease heterogeneity, our aim was to establish a new pancreatic cancer cell line. Newly established pancreatic ductal adenocarcinoma cell line Capan-26 was characterized by assessing growth rate, tumor and stem cell marker expression, colony forming efficiency, mutations of KRAS and TP53 genes, karyotype and sensitivity to drug treatment. Cell doubling time was 74 h. We detected CA19-9, CEACAM6, CD44, OCT4 and ZEB1 expression in Capan-26 cell line. Cells formed colonies in soft agar, have a deletion of KRAS exon 3 and a point mutation V172F in TP53 exon 5. They are a mixed aneuploid/polyploid population with high sensitivity to gemcitabine. Capan-26 is a unique cell line that may be used to study the mechanism of pancreatic cancer. Copyright © 2021 International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.
- Find related publications in this database (using NLM MeSH Indexing)
- Aged -
- Antigens, CD - genetics
- Antigens, CD - metabolism
- Antimetabolites, Antineoplastic - pharmacology
- Carcinoma, Pancreatic Ductal - genetics
- Carcinoma, Pancreatic Ductal - metabolism
- Carcinoma, Pancreatic Ductal - pathology
- Cell Adhesion Molecules - genetics
- Cell Adhesion Molecules - metabolism
- Cell Line, Tumor -
- Cell Proliferation - drug effects
- Cell Proliferation - genetics
- Deoxycytidine - analogs & derivatives
- Deoxycytidine - pharmacology
- Female -
- GPI-Linked Proteins - genetics
- GPI-Linked Proteins - metabolism
- Gene Expression Regulation, Neoplastic - drug effects
- Humans -
- Karyotyping -
- Mutation -
- Octamer Transcription Factor-3 - genetics
- Octamer Transcription Factor-3 - metabolism
- Pancreatic Neoplasms - genetics
- Pancreatic Neoplasms - metabolism
- Pancreatic Neoplasms - pathology
- Proto-Oncogene Proteins p21(ras) - genetics
- Tumor Suppressor Protein p53 - genetics
- Find related publications in this database (Keywords)
- Pancreatic cancer
- cell line establishment
- chemotherapy