Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Guettler, J; Forstner, D; Cvirn, G; Maninger, S; Brugger, BA; Nonn, O; Kupper, N; Pritz, E; Wernitznig, S; Dohr, G; Hutter, H; Juch, H; Isermann, B; Kohli, S; Gauster, M.
Maternal platelets pass interstices of trophoblast columns and are not activated by HLA-G in early human pregnancy.
J Reprod Immunol. 2021; 144:103280
Doi: 10.1016/j.jri.2021.103280
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- Leading authors Med Uni Graz
- Gauster Martin
- Güttler Jacqueline
- Co-authors Med Uni Graz
- Brugger Beatrice Anna
- Cvirn Gerhard
- Dohr Gottfried
- Forstner Desiree
- Hutter Heinz
- Juch Herbert
- Kupper Nadja Julia
- Maninger Sabine Elfriede
- Nonn Olivia
- Pritz Elisabeth
- Wernitznig Stefan
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- Abstract:
- In early human gestation, maternal arterial blood flow into the intervillous space of the developing placenta is obstructed by invaded trophoblasts, which form cellular plugs in uterine spiral arteries. These trophoblast plugs have recently been described to be loosely cohesive with clear capillary-sized channels into the intervillous space by 7 weeks of gestation. Here, we analysed localisation of maternal platelets at the maternal-foetal interface of human first trimester pregnancy, and tested the hypothesis whether HLA-G, which is primarily expressed by extravillous trophoblasts, affects aggregation and adhesion of isolated platelets. Immunohistochemistry of first trimester placental sections localised maternal platelets in vessel-like channels and adjacent intercellular gaps of extravillous trophoblasts in distal parts of columns. Furthermore, this localisation was confirmed by transmission electron microscopy. Neither co-incubation of HLA-G overexpressing JAR cells with isolated platelets, nor incubation with cell-derived soluble HLA-G or recombinant HLA-G affected platelet adhesion and aggregation. Our study suggests that maternal platelets flow through vessel-like channels of distal trophoblast columns and spread into adjacent lateral intercellular gaps, where platelet-derived factors could contribute to trophoblast differentiation into the invasive phenotype.
- Find related publications in this database (using NLM MeSH Indexing)
- Blood Platelets - immunology
- Cell Differentiation - immunology
- Cell Line - administration & dosage
- Coculture Techniques - administration & dosage
- Female - administration & dosage
- HLA-G Antigens - immunology, isolation & purification
- Humans - administration & dosage
- Maternal-Fetal Exchange - immunology
- Microscopy, Electron, Transmission - administration & dosage
- Placenta - blood supply, cytology, immunology, ultrastructure
- Placental Circulation - immunology
- Pregnancy - administration & dosage
- Pregnancy Trimester, First - immunology
- Primary Cell Culture - administration & dosage
- Recombinant Proteins - immunology, isolation & purification
- Trophoblasts - physiology, ultrastructure
- Find related publications in this database (Keywords)
- Early pregnancy
- Placenta
- Development
- Platelets
- HLA-G