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Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Vujić, N; Bradić, I; Goeritzer, M; Kuentzel, KB; Rainer, S; Kratky, D; Radović, B.
ATG7 is dispensable for LC3-PE conjugation in thioglycolate-elicited mouse peritoneal macrophages.
Autophagy. 2021; 17(11):3402-3407
Doi: 10.1080/15548627.2021.1874132
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- Leading authors Med Uni Graz
- Radovic Branislav
- Vujic Nemanja
- Co-authors Med Uni Graz
- Bradic Ivan
- Göritzer Madeleine
- Kratky Dagmar
- Küntzel Katharina Barbara
- Rainer Silvia
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- Abstract:
- Thioglycolate-elicited macrophages exhibit abundant conjugation of LC3 with PE (LC3-II). Among other autophagy-related (ATG) proteins, it is proposed that, like in yeast, both ATG5 and ATG7 are essential for LC3 conjugation. Using atg5-deficient (-/-) and atg7-/-macrophages, we provide evidence that loss of ATG5 but not of ATG7 resulted in LC3-II depletion. Accumulation of LC3-II in elicited atg7-/- macrophages in response to bafilomycin A1 validated these data. Furthermore, complete loss of ATG3 in atg7-/- macrophages demonstrated that ATG7 and ATG3 are dispensable for LC3-PE conjugation. In contrast to thioglycolate-elicited macrophages, naïve peritoneal and bone marrow-derived atg7-/- macrophages exhibited no LC3-II, even under inflammatory stimuli in vitro. Hence, the macrophage metabolic status dictates the level of LC3-PE conjugation with a supportive but nonessential role of ATG7, disclosing the eukaryotic exception from the LC3 lipidation model based on yeast data. Abbreviations: ATG: autophagy-related; BM: bone marrow; MAP1LC3/LC3: microtubule-associated protein 1 light chain 3; PE: phosphatidylethanolamine.
- Find related publications in this database (Keywords)
- ATG3
- ATG5
- ATG7
- LC3 lipidation
- LC3-II
- macrophages