Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Rauter, T; Depaoli, MR; Bischof, H; Graier, WF; Malli, R.
Metabolic Profiling of Single Cancer Cells Using Mitochondrial ATP Probes.
STAR Protoc. 2020; 1(2): 100048-100048.
Doi: 10.1016/j.xpro.2020.100048
[OPEN ACCESS]
Web of Science
PubMed
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- Leading authors Med Uni Graz
- Depaoli Maria Rosa
- Malli Roland
- Rauter Thomas
- Co-authors Med Uni Graz
- Bischof Helmut
- Graier Wolfgang
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- Abstract:
- The metabolic activity of cells is interrelated with cell signaling, functions, and fate. Uncontrolled cancer cell proliferation requires metabolic adaptations. Research focusing on understanding the characteristics of cell metabolism is crucial for the development of novel diagnostic and therapeutic strategies. Here, we describe protocols for the ATP profiling of single cancer cells by fluorescence live-cell imaging. In response to distinct metabolic inhibitions, we record individual mitochondrial ATP dynamics using established Förster resonance energy transfer-based genetically encoded fluorescent ATP probes. For complete details on the use and execution of this protocol, please refer to Depaoli et al. (2018). © 2020 The Author(s).