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Obasi, A; Nwachukwu, S; Ugoji, E; Kohler, C; Göhler, A; Balau, V; Pfeifer, Y; Steinmetz, I.
Extended-Spectrum β-Lactamase-Producing Klebsiella pneumoniae from Pharmaceutical Wastewaters in South-Western Nigeria.
Microb Drug Resist. 2017; 23(8): 1013-1018. Doi: 10.1089/mdr.2016.0269
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Co-authors Med Uni Graz: Steinmetz Ivo
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Abstract:: Emergence and spread of Klebsiella pneumoniae isolates producing extended-spectrum β-lactamases (ESBLs) present a major threat to public health. In this study, we characterized β-lactam-resistant K. pneumoniae isolates from six wastewater samples obtained from two pharmaceutical industries located in Lagos and Ogun States, Nigeria. Bacteria were isolated by using MacConkey agar; species identification and antibacterial susceptibility testing were performed by Vitek 2. Etest was used for ESBL phenotype confirmation. The presence of β-lactamase genes was investigated by PCR and sequencing. Bacterial strain typing was done by XbaI-macrorestriction and subsequent pulsed-field gel electrophoresis (PFGE) as well as multilocus sequence typing (MLST). Thirty-five bacterial species were isolated from the six samples; among them, we identified seven K. pneumoniae isolates with resistance to β-lactams and co-resistance to fluoroquinolones, aminoglycosides, and folate pathway inhibitors. The ESBL phenotype was confirmed in six K. pneumoniae isolates that harbored ESBL genes bla_CTX-M-15 (n = 5), bla_SHV-2 (n = 1), and bla_SHV-12 (n = 1). PFGE and MLST analysis revealed five clones belonging to four sequence types (ST11, ST15, ST37, ST101), and clone K. pneumoniae-ST101 was present in the wastewater samples from two different pharmaceutical industries. Additionally performed conjugation assays confirmed the location of β-lactamase genes on conjugative plasmids. This is the first confirmation of K. pneumoniae isolates producing CTX-M-15-ESBL from pharmaceutical wastewaters in Nigeria. The co-resistance observed might be a reflection of the different drugs produced by these industries. Continuous surveillance of the environmental reservoirs of multidrug-resistant bacteria is necessary to prevent their further spread.
Find related publications in this database (using NLM MeSH Indexing): Anti-Bacterial Agents - pharmacology; Bacterial Proteins - metabolism; Bacterial Typing Techniques - methods; Drug Industry - methods; Drug Resistance, Multiple, Bacterial - drug effects; Klebsiella pneumoniae - drug effects; Klebsiella pneumoniae - metabolism; Molecular Epidemiology - methods; Nigeria -; Waste Water - microbiology; beta-Lactamases - metabolism; beta-Lactams - pharmacology
Find related publications in this database (Keywords): CTX-M-15; SHV-12; SHV-2; multidrug resistance; conjugative plasmids