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SHR Neuro Cancer Cardio Lipid Metab Microb
Pusceddu, I; Herrmann, M; Kirsch, SH; Werner, C; Hübner, U; Bodis, M; Laufs, U; Wagenpfeil, S; Geisel, J; Herrmann, W.
Prospective study of telomere length and LINE-1 methylation in peripheral blood cells: the role of B vitamins supplementation.
Eur J Nutr. 2016; 55(5):1863-1873
Doi: 10.1007/s00394-015-1003-1
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- Co-authors Med Uni Graz
- Herrmann Markus
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- Abstract:
- Deficiencies of folate, vitamins B12 and D are common age-related conditions. Vitamin B12 and folate are necessary for DNA methylation. Telomeres appear to be regulated by DNA methylation. Here, we study the effect of B vitamins supplementation on telomere length and global DNA methylation in a prospective study. In total, 60 elderly subjects were supplemented for 1 year with either vitamin B12, B6, folate, vitamin D and calcium (group A n = 31) or only vitamin D and calcium (group B n = 29). LINE-1 methylation, relative telomere length (T/S), vitamin B12, folate, homocysteine (tHcy) , 5-methyltetrahydrofolate (5-methylTHF), S-adenosylhomocysteine (SAH), S-adenosylmethionine (SAM), cystathionine and vitamin D were quantified before and after supplementation. At baseline, tHcy was high, vitamin D was low, and T/S did not differ between groups A and B. Vitamin supplementation increased LINE-1 methylation in group A at site 317 but reduced LINE-1 methylation in group B at site 327. There was no correlation between T/S and LINE-1 methylation at baseline. Multiple backward regression analysis revealed baseline tHcy and 5-methylTHF are significant predictors of T/S. After supplementation in group B but not in group A, LINE-1 methylation correlated inversely with T/S, and LINE-1 methylation variation was an independent predictor of T/S variation. B vitamins decreased tHcy significantly in group A. Multiple backward regression analysis showed 5-methylTHF in group A and tHcy in group B were significant predictors for LINE-1 methylation. At baseline, the lower LINE-1 methylation observed in subjects with 5-methylTHF >10 nmol/l was in agreement with a reduced methyl group transfer due to a lower SAM formation. In group B, an increase in telomere length was correlated with lower LINE-1 methylation. Subjects with hyperhomocysteinemia >12 µmol/L had compared to those with normal tHcy a reduced LINE-1 methylation accompanied by a higher SAM and SAH (that inhibits demethylation of SAM) as well as lower 5-methylTHF. Additionally, subjects with tHcy > 12 µmol/L had longer telomeres when compared with subjects having tHcy < 12 µmol/L. The results suggest a possible effect of B vitamins for telomere biology in blood cells. Suboptimal B vitamins status and hyperhomocysteinemia are associated with altered DNA methylation and telomere length. These data have to be confirmed in future studies.
- Find related publications in this database (using NLM MeSH Indexing)
- Aged -
- Blood Cells - drug effects
- Calcium - administration & dosage
- Calcium - blood
- Cross-Sectional Studies -
- DNA Methylation - drug effects
- Dietary Supplements -
- Female -
- Folic Acid - administration & dosage
- Folic Acid - blood
- Homocysteine - blood
- Humans -
- Hyperhomocysteinemia - drug therapy
- Linear Models -
- Long Interspersed Nucleotide Elements - genetics
- Male -
- Middle Aged -
- Prospective Studies -
- S-Adenosylhomocysteine - blood
- S-Adenosylmethionine - blood
- Telomere - ultrastructure
- Tetrahydrofolates - blood
- Vitamin B 12 - administration & dosage
- Vitamin B 12 - blood
- Vitamin B 6 - administration & dosage
- Vitamin B 6 - blood
- Vitamin B Complex - administration & dosage
- Vitamin B Complex - blood
- Vitamin D - administration & dosage
- Vitamin D - blood
- Find related publications in this database (Keywords)
- B vitamins
- Telomere length
- DNA methylation