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SHR Neuro Cancer Cardio Lipid Metab Microb
Liu, Q; Bruns, H; Schultze, D; Xue, Y; Zorn, M; Flechtenmacher, C; Straub, BK; Rauen, U; Schemmer, P.
HTK-N, a modified HTK solution, decreases preservation injury in a model of microsteatotic rat liver transplantation.
Langenbecks Arch Surg. 2012; 397(8):1323-1331
Doi: 10.1007/s00423-012-1022-9
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- Leading authors Med Uni Graz
- Schemmer Peter
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- Abstract:
- Ischemia/reperfusion injury is an obstacle especially in steatotic livers, including those with steatosis induced by acute toxic stress. Recently, a modified histidine-tryptophan-ketoglutarate (HTK) solution, HTK-N, has been developed. This solution contains N-acetylhistidine, amino acids, and iron chelators. This study was designed to test the effects of HTK-N on preservation injury to rat livers after acute toxic injury. Microvesicular steatosis was induced by a single dose of ethanol (8 g/kg BW). Livers were harvested and stored at 4 °C for 8 h with HTK or HTK-N before transplantation. Tissue and blood samples were taken at 1, 8, and 24 h after reperfusion to compare serum liver enzymes (aspartate aminotransferase, alanine aminotransferase, and lactate dehydrogenase), standard histology, and immunohistochemistry for myeloperoxidase (MPO), caspase-3, and inducible nitric oxide synthase. Survival was compared after 1 week. For statistics, Analysis of Variance and t test were used. HTK-N improved survival from 12.5% in HTK to 87.5% (p < 0.05). Furthermore, liver enzymes were decreased to 2-75% of HTK values (p < 0.05). Necrosis and leukocyte infiltration and MPO, caspase-3, and iNOS expression after transplantation were decreased (p < 0.05). This study demonstrates that HTK-N protects liver grafts with microvesicular steatosis caused by acute toxic injury from cold ischemic injury better than standard HTK most likely via inhibition of hypoxic injury and oxidative stress and amelioration of the inflammatory reaction occurring upon reperfusion.
- Find related publications in this database (using NLM MeSH Indexing)
- Alanine Transaminase - blood
- Animals -
- Aspartate Aminotransferases - blood
- Cold Temperature -
- Ethanol - toxicity
- Fatty Liver - pathology
- Fatty Liver - surgery
- Female -
- Glucose - chemistry
- Histidine - analogs & derivatives
- Histidine - analysis
- Iron Chelating Agents -
- L-Lactate Dehydrogenase - blood
- Liver - blood supply
- Liver - pathology
- Liver Transplantation - mortality
- Liver Transplantation - pathology
- Mannitol - chemistry
- Microvessels - drug effects
- Microvessels - pathology
- Organ Preservation Solutions -
- Potassium Chloride - chemistry
- Procaine - chemistry
- Rats -
- Rats, Sprague-Dawley -
- Survival Rate -
- Find related publications in this database (Keywords)
- Liver transplantation
- Steatosis
- Cold ischemia
- Organ preservation
- Reperfusion injury
- Preservation solution