Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Schindler, G; Kincius, M; Liang, R; Backhaus, J; Zorn, M; Flechtenmacher, C; Gebhard, MM; Büchler, MW; Schemmer, P.
Fundamental efforts toward the development of a therapeutic cocktail with a manifold ameliorative effect on hepatic ischemia/reperfusion injury.
Microcirculation. 2009; 16(7):593-602
Doi: 10.1080/10739680903110779
Web of Science
PubMed
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- Leading authors Med Uni Graz
- Schemmer Peter
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- Abstract:
- Ischemia/reperfusion injury is mediated by various mechanisms. The present study was designed to evaluate the effect of a multiple pharmacological approach toward ischemia/reperfusion injury reduction. The left liver lobe of Sprague-Dawley rats underwent normothermic ischemia for 90 minutes after a cocktail (glycine, taurine, alanine, arginine, and prednisolon) intravenous administration. Controls received normal saline. Liver injury (transaminases, histology) and cellular activation [Kupffer cell phagocytosis, production of tumor necrosis factor-alpha (TNFalpha), and prostaglandin E-2 (PGE(2))], as well as microcirculation and leukocyte-endothelial interaction (in vivo microscopy), were assessed. Whereas in controls a substantial increase of aspartate aminotransferase, alanine aminotransferase, and lactate dehydrogenase to 2,341+/-477, 1,442+/-262, and 1,973+/-73 U/L, respectively, was measured eight hours after reperfusion, the cocktail significantly reduced the increase of enzymes by 33-80% (P<0.05). Further, necrosis, index of liver damage, and index of leukocyte infiltration significantly decreased after the cocktail (P<0.05). Moreover, the cocktail improved acinar and sinusoidal perfusion, while the sinusoidal diameters, leukocyte-endothelial interaction, Kupffer cell phagocytic activity, and TNFalpha/PGE(2) serum levels were significantly reduced, the latter by 86% and to 1.64-fold, respectively. This study depicts that multifaceted pharmacological tackling of ischemia/reperfusion injury is feasible and protects rat liver tissue from warm ischemia/reperfusion injury.
- Find related publications in this database (using NLM MeSH Indexing)
- Alanine - therapeutic use
- Animals -
- Arginine - therapeutic use
- Chemotaxis, Leukocyte -
- Clinical Enzyme Tests -
- Drug Therapy, Combination -
- Glycine - therapeutic use
- Liver Circulation -
- Liver Diseases - drug therapy
- Liver Diseases - enzymology
- Liver Diseases - pathology
- Necrosis -
- Phagocytosis -
- Prednisolone - therapeutic use
- Rats -
- Rats, Sprague-Dawley -
- Reperfusion Injury - drug therapy
- Taurine - therapeutic use
- Treatment Outcome -
- Find related publications in this database (Keywords)
- ischemia
- reperfusion injury
- liver
- microcirculation
- cocktail
- glycine
- taurine
- arginine
- alanine
- prednisolon