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SHR Neuro Cancer Cardio Lipid Metab Microb

Macheiner, T; Fengler, VH; Agreiter, M; Eisenberg, T; Madeo, F; Kolb, D; Huppertz, B; Ackbar, R; Sargsyan, K.
Magnetomitotransfer: An efficient way for direct mitochondria transfer into cultured human cells.
Sci Rep. 2016; 6(4):35571-35571 Doi: 10.1038/srep35571 [OPEN ACCESS]
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Leading authors Med Uni Graz: Macheiner Tanja; Sargsyan Karine
Co-authors Med Uni Graz: Ackbar Richard; Agreiter Marlene; Fengler Vera; Huppertz Berthold; Kolb Dagmar
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Abstract:: In the course of mitochondrial diseases standard care mostly focuses on treatment of symptoms, while therapeutic approaches aimed at restoring mitochondrial function are currently still in development. The transfer of healthy or modified mitochondria into host cells would open up the possibilities of new cell therapies. Therefore, in this study, a novel method of mitochondrial transfer is proposed by anti-TOM22 magnetic bead-labeled mitochondria with the assistance of a magnetic plate. In comparison to the passive transfer method, the magnetomitotransfer method was more efficient at transferring mitochondria into cells (78-92% vs 0-17% over 3 days). This transfer was also more rapid, with a high ratio of magnetomitotransferred cells and high density of transferred mitochondria within the first day of culture. Importantly, transferred mitochondria appeared to be functional as they strongly enhanced respiration in magnetomitotransferred cells. The novel method of magnetomitotransfer may offer potential for therapeutic approaches for treatment of a variety of mitochondria-associated pathologies, e.g. various neurodegenerative diseases.
Find related publications in this database (using NLM MeSH Indexing): Cell Line -; Fibroblasts - cytology; Fibroblasts - metabolism; Humans -; Magnetic Fields -; Mitochondria - metabolism; Mitochondria - transplantation