Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Erttmann, SF; Bast, A; Seidel, J; Breitbach, K; Walther, R; Steinmetz, I.
PGD2 and PGE2 regulate gene expression of Prx 6 in primary macrophages via Nrf2.
Free Radic Biol Med. 2011; 51(3):626-640
Doi: 10.1016/j.freeradbiomed.2011.05.022
Web of Science
PubMed
FullText
FullText_MUG
- Co-authors Med Uni Graz
- Steinmetz Ivo
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- Peroxiredoxin 6 (Prx 6) is a bifunctional enzyme with both glutathione peroxidase and acidic Ca(2+)-independent phospholipase A(2) activities. We have recently shown that exposure of murine bone marrow-derived macrophages to LPS and IFN-γ leads to induction of COX-2 expression and secretion of PGE(2), up-regulating Prx 6 mRNA levels. This study was designed to investigate various prostaglandins (PGs) for their ability to induce gene expression of Prxs, in particular Prx 6, and to determine the underlying regulatory mechanisms. We provide evidence that both conventional and cyclopentenone PGs enhance Prx 6 mRNA expression. Treatment with either activators or inhibitors of adenylate cyclase as well as cAMP analogs indicated that Prx 6 gene expression is regulated by adenylate cyclase in response to PGD(2) or PGE(2). Furthermore, our study revealed that JAK2, PI3K, PKC, and p38 MAPK contribute to the PGD(2)- or PGE(2)-dependent Prx 6 induction. Using stimulated macrophages from Nrf2-deficient mice or activators of Nrf2 and PPARγ, we found that Nrf2, but not PPARγ, is involved in the PG-dependent increase in Prx 6 mRNA expression. In summary, our data suggest multiple signaling pathways of Prx 6 regulation by PGs and identified Nrf2 as a critical player mediating transcriptional induction. Copyright © 2011 Elsevier Inc. All rights reserved.
- Find related publications in this database (using NLM MeSH Indexing)
- Adenylyl Cyclases - metabolism
- Animals -
- Cells, Cultured -
- Cyclic AMP - analogs & derivatives
- Cyclic AMP - pharmacology
- Dinoprostone - genetics
- Dinoprostone - metabolism
- Gene Expression Regulation - drug effects
- Janus Kinase 2 - metabolism
- MAP Kinase Signaling System -
- Macrophages - drug effects
- Macrophages - metabolism
- Macrophages - pathology
- Mice -
- Mice, Inbred C57BL -
- Mice, Knockout -
- NF-E2-Related Factor 2 - genetics
- NF-E2-Related Factor 2 - metabolism
- Peroxiredoxin VI - genetics
- Peroxiredoxin VI - metabolism
- Phosphatidylinositol 3-Kinases - metabolism
- Prostaglandin D2 - genetics
- Prostaglandin D2 - metabolism
- Protein Kinase C - metabolism
- Transcriptional Activation - genetics
- Find related publications in this database (Keywords)
- Adenylate cyclase
- JAK2
- Macrophage
- MAPK
- Nrf2
- Peroxiredoxin
- PI3K
- PKC
- Prostaglandin
- Free radicals