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Selected Publication:
Nimtz, M; Wray, V; Domke, T; Brenneke, B; Häussler, S; Steinmetz, I.
Structure of an acidic exopolysaccharide of Burkholderia pseudomallei.
Eur J Biochem. 1997; 250(2):608-616
Doi: 10.1111/j.1432-1033.1997.0608a.x
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- Leading authors Med Uni Graz
- Steinmetz Ivo
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- Abstract:
- A recently described water-soluble exopolysaccharide of Burkholderia pseudomallei recognized by the IgG 1 monoclonal antibody 3015 [Steinmetz, I., Rohde, M. & Brenneke, B. (1995) Infect. Immun. 63, 3959-3965] was isolated by repetitive ethanol-precipitation steps and by anion-exchange chromatography. The structure of the polysaccharide was determined by a combination of chemical-derivatization and mass-spectrometric techniques (compositional and methylation analysis, GC/MS, and electrospray-ionization-MS/MS of reduced and permethylated hydrolytic fragments), and two-dimensional 1H-NMR methods (COSY, TOCSY and NOESY) and confirmed by isolation and structural characterization of the depolymerized repeating unit of the polysaccharide. The combined structural data established a linear tetrasaccharide repeating unit consisting of three galactose residues, one bearing a 2-linked O-acetyl group, and a 3-deoxy-D-manno-2-octulosonic acid residue. [-->3)-beta-D-Galp2Ac-(1-->4)-alpha-D-Galp-(1-->3)-beta-D-Galp-(1- ->5)-beta-Kdo-(2-->]n
- Find related publications in this database (using NLM MeSH Indexing)
- Burkholderia pseudomallei - chemistry
- Polysaccharides, Bacterial - chemistry
- Polysaccharides, Bacterial - isolation & purification
- Find related publications in this database (Keywords)
- Burkholderia pseudomallei
- exopolysaccharide