Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Wehmhöner, D; Häussler, S; Tümmler, B; Jänsch, L; Bredenbruch, F; Wehland, J; Steinmetz, I.
Inter- and intraclonal diversity of the Pseudomonas aeruginosa proteome manifests within the secretome.
J Bacteriol. 2003; 185(19):5807-5814
Doi: 10.1128/JB.185.19.5807-5814.2003
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- Co-Autor*innen der Med Uni Graz
- Steinmetz Ivo
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- Abstract:
- The proteomes of cultured Pseudomonas aeruginosa isolates from chronically infected cystic fibrosis (CF) lungs were compared by using genetically divergent clones and isogenic morphotypes of one strain. Cellular extracts gave very similar protein patterns in two-dimensional gels, suggesting that the conserved species-specific core genome encodes proteins that are expressed under standard culture conditions in vitro. In contrast, the protein profiles of extracts of culture supernatants were dependent on the growth phase, and there were significant differences between clones. The profiles also varied within clonally related morphotypes from one CF patient, including a hyperpiliated small-colony variant. Mass spectrometry revealed that this variant overexpressed proteins secreted by the type I secretion system (including proteins involved in iron acquisition) and by the type III secretion system. Furthermore, the proteins in the supernatant extracts from the small-colony variant which were recognized by sera from different CF patients varied greatly. We concluded that the secretome expression is a sensitive measure of P. aeruginosa strain variation.
- Find related publications in this database (using NLM MeSH Indexing)
- Antibodies, Bacterial - blood
- Bacterial Proteins - genetics
- Bacterial Proteins - immunology
- Bacterial Proteins - secretion
- Chronic Disease -
- Culture Media, Conditioned -
- Cystic Fibrosis - microbiology
- Electrophoresis, Gel, Two-Dimensional -
- Gene Expression Regulation, Bacterial -
- Genetic Variation -
- Humans -
- Proteome -
- Pseudomonas Infections - microbiology
- Pseudomonas aeruginosa - classification
- Pseudomonas aeruginosa - genetics
- Pseudomonas aeruginosa - growth & development
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization -