Medizinische Universität Graz - Research portal
Selected Publication:
Hübner, I; Steinmetz, I; Obst, U; Giebel, D; Bitter-Suermann, D.
Rapid determination of members of the family Enterobacteriaceae in drinking water by an immunological assay using a monoclonal antibody against enterobacterial common antigen.
Appl Environ Microbiol. 1992; 58(9):3187-3191
Doi: 10.1128/aem.58.9.3187-3191.1992
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- Co-authors Med Uni Graz
- Steinmetz Ivo
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- Abstract:
- An immunological method for the detection of members of the family Enterobacteriaceae in drinking water was developed. The method was based on a sandwich enzyme-linked immunosorbent assay (ELISA) with monoclonal antibody immunoglobulin G2a 898 against enterobacterial common antigen. The enterobacterial common antigen sandwich ELISA combined with selective preenrichment culture could be performed in only 24 h. Six hundred sixty-eight water samples from a variety of German public water supplies were screened to verify the effectiveness of the new method. Ninety-eight percent of the results obtained by the immunological method could be confirmed by conventional microbiological methods. The immunological method proved to be considerably faster and more specific and sensitive than the standard method specified by the German drinking water regulations.
- Find related publications in this database (using NLM MeSH Indexing)
- Antibodies, Bacterial -
- Antibodies, Monoclonal -
- Antigens, Bacterial - immunology
- Enterobacteriaceae - classification
- Enterobacteriaceae - immunology
- Enterobacteriaceae - isolation & purification
- Enzyme-Linked Immunosorbent Assay - methods
- Water Microbiology -
- Water Supply -