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Selected Publication:
Heitmann, S; Tschernig, T; Larbig, M; Steinmetz, I; Hedrich, HJ; Pabst, R.
Immunohistological characterization of leukocytes in the lungs of healthy mice and after bacterial intratracheal infection.
Lab Anim. 1999; 33(3):288-294
Doi: 10.1258/002367799780578147
Web of Science
PubMed
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- Co-authors Med Uni Graz
- Steinmetz Ivo
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- Abstract:
- Leukocytes in the peripheral lung parenchyma of mice have not been characterized histologically during bacterial infection. The aim of this study was to investigate (a) the immunohistological characteristics of healthy murine lungs and (b) the cell kinetics during acute inflammation. BALB/c and MF1 mice were examined; as well as transgenic mice with the gene defect of cystic fibrosis (CF) in the airways as an animal model for this disease. MF1 mice served as controls for the transgenic animals. Lavaged and perfused lungs were snap frozen. B and T lymphocytes, CD4+ and CD8+ cells, dendritic cells, neutrophils and a subset of macrophages were enumerated on cryostat lung sections. The lung tissue and bronchoalveolar lavage (BAL) of BALB/c mice, infected intratracheally with Haemophilus influenzae type b (Hib), were studied at different time points after infection. In the lungs of healthy mice, including CF mice, the largest population was that of T cells, CD4+ cells being always more frequent than CD8+ cells. During acute inflammation the number of neutrophils in the lung parenchyma and BAL increased strongly within the first hours after bacterial instillation and reached baseline levels within one week. This study provides a semi-quantitative analysis of immunocompetent cells in normal and infected murine lung tissue. Differences in cell numbers are found between different strains. Moreover, the cellular reaction during Hib infection in mouse lungs is dominated by neutrophils, as expected in a primary immune response. In uninfected CF mice the numbers and distribution of immune cells in the lung tissue are normal, indicating that the cellular defense is adequate.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Bacterial Infections - pathology
- Bronchi - pathology
- Cystic Fibrosis Transmembrane Conductance Regulator - genetics
- Female -
- Granulocytes - pathology
- Immunohistochemistry -
- Kinetics -
- Leukocytes - pathology
- Lung - pathology
- Macrophages, Alveolar - pathology
- Male -
- Mice -
- Mice, Inbred BALB C -
- Mice, Transgenic -
- Mutation -
- Neutrophils - pathology
- Pneumonia - microbiology
- Pneumonia - pathology
- T-Lymphocytes - pathology
- Trachea - microbiology
- Find related publications in this database (Keywords)
- mouse lungs
- leukocytes
- immunohistology
- cystic fibrosis
- bacterial infection