Medizinische Universität Graz - Research portal
Selected Publication:
Steinmetz, I; Rheinheimer, C; Bitter-Suermann, D.
Rapid identification of legionellae by a colony blot assay based on a genus-specific monoclonal antibody.
J Clin Microbiol. 1992; 30(4):1016-1018
Doi: 10.1128/JCM.30.4.1016-1018.1992
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- Steinmetz Ivo
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- Abstract:
- We recently developed a monoclonal antibody immunoglobulin G2a (2125) recognizing a genus-specific epitope on the 60-kDa heat shock protein of all Legionella species. In the current study, this antibody was used in a colony blot enzyme-linked immunosorbent assay for the rapid identification of Legionella cultures on agar plates. The whole protocol was completed in less than 2 h. All 59 Legionella species and serogroups that were tested gave a positive signal. No unspecific reactions with nonlegionellae were observed. This test is a rapid procedure for the identification of legionellae growing on agar medium to the genus level.
- Find related publications in this database (using NLM MeSH Indexing)
- Antibodies, Bacterial -
- Antibodies, Monoclonal -
- Antigens, Bacterial -
- Bacteriological Techniques -
- Epitopes -
- Evaluation Studies as Topic -
- Heat-Shock Proteins - immunology
- Humans -
- Legionella - growth & development
- Legionella - immunology
- Legionella - isolation & purification
- Species Specificity -